| Background:The EB virus is a kind of complex genomes of human herpes virus, over90%of the world’s people had the virus infection. EB virus on human universal susceptibility and with a variety of human diseases especially malignant tumor disease is closely related to. The EB virus in the body infection in nasopharyngeal carcinoma (NPC) in the diagnosis, staging, prognosis and monitoring the recurrence and metastasis after radiotherapy is important. Some researchers proposed that the EB virus was one of the major causative factors of nasopharyngeal carcinoma. EB virus and nasopharyngitis also have close correlation, studies have found that EB virus infected with EB virus negative had higher prevalence of nasopharyngitis.Objective:This research through the pharmacodynamic immune experiment, objective evaluation of Qi Ju Teabag Pharmacodynamics on nonspecific immune function of mice. Based on EB virus infection:Observation of clinical efficacy, and the control group did not be used any clinical intervention group for comparison, observation of chrysanthemum tea on EB virus infection treatment effects, with a view to TCM treatment of EB virus infection to provide an effective and practical means of treatment, on EB virus infection prevention and treatment and improve the treatment of traditional Chinese medicine in the treatment of EB virus infection will have important significance.Methods:1. pharmacodynamic experiment:mouse weighing, numbers, were randomly divided into experimental group, standard control group and blank control group, the experimental group was divided into high dose group, middle dose group, low dose group. According to the experimental scheme of gavage administration, injection of chicken red cells suspension, mice were sacrificed, extraction of peritoneal fluid in0.5m L, then droped in a clean glass slides, each piece of0.5m L, each mouse had2pieces. And then they were placed on the pad with wet gauze enamel boxes,37degrees C were incubated in half an hour. Slide water rushed out of the adherent cells, and then natural dried the glass slideses. Using acetone and methanol solution (1:1) fixed for5minutes. Giem SA-W righ t mixed staining in3～5minutes, tap water was used to rinse them, then they were dryed. We counted200macrophage phagocytic index by oil mirror view, and calculated phagocytic index (the phagocytic index=be swallowed chicken RBC/200macrophage) and phagocytic percentage (phagocytic percentage=swallowing chicken red blood cells of macrophage/200macrophage). We recorded all data for statistical analysis, variance analysis.2. Clinical curative effect observation:After inclusion criteria and exclusion criteria were formulated, the EB virus or EB virus infection combined with Qi deficiency and heat nasopharyngitis patients using look-up table of random numbers randomly were assigned into two groups, one group of chrysanthemum tea was the treatment group, they oraled the teabags,2bags for one time, method was drinking after boiling water soaking them15minutes,2times a day; a group was the control group without any clinical intervention group. After3weeks, based the review of the EB virus, EB virus negative effect was observated.3. Statistical analysis:count data by using chi-square test, measurement data using t test, all data were entered into the computer database, using SPSS13. Ostatistical software for statistical analysis, and testing standards for a=0.05. The two groups of patients’EB virus seroconversion rates before and after treatment were compared, and compared between groups, then we got the comparison of curative effect between the two groups.Result:1.Pharmacodynamic experimentStatistics of phagocytic rate (percentage of phagocytosis of macrophages phagocytosis of chicken red blood cell count/200macrophages) and the phagocytic index (the phagocytic index=be swallowed chicken RBC/200macrophages), between the5groups by analysis of variance, variance homogeneity test of P>0.05, homogeneity of variance, the groups could be compared. Comparison between groups, P<0.05, phagocytosis rate and phagocytic index contrast from experimental group, blank control group and standard control group was different, the experimental group and the standard control group and blank control group compared with difference, middle dose group and high dose group, low dose group, standard control group and blank control group respectively the difference was statistically significant, indicating, Qi Ju Teabag had enhanced non-specific immunological function in mice.2.Clinical curative effect observationAll cases were from2011March to2012April, the first hospital of Guangzhou University of Chinese Medicine ENT out-patient clinic, the all56patients were divided into two groups randomly. A group of Qi Ju Teabag treatment group of26cases,18cases complicated with sinusitis associated symptoms,8cases with symptoms. Another group of blank control group had30cases, with the nose pharyngitis related symptoms in22cases, without amalgamative symptom in8cases. According to the requirements of medication follow-up review. By statistical method statistical analysis, treatment group and control group in the total score before treatment, gender, age, tobacco and alcohol addiction, were not difference, P>0.05, because of the basic balance of information among two groups, they could be compared. In this study26cases in the treatment group patients taking Qi Ju Teabag period had not obvious adverse reaction, and after3weeks the review of serum EB virus, of which12were overcast, cloudy rate of46.2%for the control group, serum EB virus negative review in5patients, negative rate of16.7%, according to a chi-square test, the comparisons between the two groups EB virus infection negative rate had statistical significance, P<0.05. The treatment group of18cases with nasal pharyngitis related symptoms check serum EB virus in9cases negative, negative rate was50%. In8patients without associated with symptoms of3cases of cloudy, overcast rate was37.5%. In the control group, with the nose pharyngitis related symptoms in22cases in3cases of cloudy, overcast rate was16.7%. In8patients without associated with symptoms of2cases of cloudy, overcast rate was25%. The chi-square test, comparison of the treatment group and the control group with symptomatic and asymptomatic seroconversion was not statistically significant, P>0.05. After treatment, between the two groups, the total integral difference, the difference of main and secondary symptoms and signs in the difference were compariedby t Inspection, P<0.01. They were different significantly.Conclusion:Qi Ju Teabag could enhance non-specific immunological function in mice in pharmacodynamics, promoting the EB virus becoming negative and improving the clinical symptoms of the patients with EB infection.Qi Ju Teabag was a pure Chinese medicinal preparation with high safetyandconvenient taking and carrying, so that it could be widely popularized in clinic. |
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