Quantitative Analysis And Pharmacokinetic Study Of Pogostone In Pogostemonis

ObjectiveThe thesis deals with the study on Pogostone, the effective component of Pogostemon cablin, including the quantitative analysis and pharmacokinetics study in mice. To establish quantitative control methods for Pogostemon cablin, and to realize the intracorporal process of Pogostone in mice, so as toquality of this medical materials effectively and apply data for the clinical practice of Pogostemon cablin.Methods(1) After backflow extraction with ethanol, the sample was separated at30℃on a Alltech C18(250×4.6mm,5μm) eluted with acetonitrile and phosphoric acid. The detection wavelength was310nm, and the velocity of flow was0.8mL/min.(2) We used Acetonitrile precipitation to prepare the plasma samples, and used HPLC to establish a method for determination of pogostone. Chromatographic column for Alltech C18column (250×4.6mm,5μm), the mobile phase:acetonitrile-0.2%phosphoric acid (V/V,65:35),detected wavelength:310nm, Velocity:1.0mL/min, Column temperature:30℃.(3) We carried out a research on pharmacokinetics study in mice based on the established method,and to analyze the data using the DAS2.1.1software.Results(1) A method has been established for determination of Pogostone in Pogostemon cablin and aAnalysed the quality of11batches of Pogostemon cablin. The linear regression for pogostone was obtained in the range of25ng-300ng,. The method was simple, repetitive stability,and the recovery above99%. The content of pogostone in11batches of Patchouli oil range from1.4-5.7mg/g.(2) A method has been established for determination of pogostone in biological plasma sample,the linear regression was well in the range of0.2and40μg·mL-1, the method is simple, repetitive stability,and the recovery above85%..(3) The pharmacokinetic process in mice afte intravenous injection and oral administration Pogostone was to fit linear relationship,the Cmax and AUC in proportion to the dose;Tmax of injection and oral administration were2min and25min.t1/2of intravenous injection were31.30,12.04,13.20min,as t1/2of oral administration were53.31and51.96min.The results point out that the pharmacokinetic process of Pogostone in mice to meet the three-compartment model in,as the oral administration meet to the two-compartment model.The average relative bioavailability is76.67%.Conclusions(1) The result shows that the methods established in this paper is simple and rapid, precise and reliable, can be used in determining the content of Pogostemon cablin and quality control of Pogostone.(2)The linear regression for pogostone in mice plasma was well in the range of0.2and40μg/mL-1, the average recovery, precision and stability was satisfactory.(3) The Cmax and AUC in proportion to the dose,as the Tmas and t1/2with no relevance to dose.The results of bioavailability suggested that Pogostone absorbed well in mice.