Syndrome Type Of Esophageal Cancer Cells With Chemotherapy In Patients Experimental Study Of Drug Sensitivity

Esophageal cancer is one of common gastrointestinal clinic in China have high morbidityand mortality, a serious threat to people’s health. Treatment of esophageal cancer are surgery,radiotherapy, chemotherapy, biological therapy and traditional Chinese medicine and othermethods; surgery, although the preferred treatment, but many patients are already in advancedwhen found, lost the chance of surgery, chemotherapy remains the main result one of themeans of treatment. At present each report cases of esophageal cancer are more efficient, butmost scholars reported effective rate low. In recent years, the treatment of esophagealIntegrative Medicine has been widely appreciated. Chinese medicine to reduce side effects ofchemotherapy, enhanced sensitivity to improve the efficacy and reversal of chemotherapyresistance has its unique advantages. Therefore, the use of MTT assay clinical sensitivity tochemotherapeutic drugs commonly used in esophageal cancer, provide the basis for clinicaldrug selection, study various TCM syndrome in patients with esophageal cancer cells tochemotherapeutic drugs and in vitro correlation between sensitivity by identifyingdiseases combined with the syndrome to develop the best individualized Integrative Medicinefor seeking the ideal clinical efficacy.Objective: This study of60patients with newly diagnosed patients with primaryesophageal cancer, the TCM syndrome; application MTT method esophageal cancer patientswith different syndromes of esophageal cancer cells to chemotherapeutic drugs commonlyused clinical5in vitro drug sensitivity analysis of different pathological types of esophagealcancer patients to chemotherapy sensitivity differences and classification of TCM withchemotherapy correlation between drug sensitivity.Methods:①through General Surgery, Henan Tumor Hospital from October2009toDecember2010to treat patients with esophageal cancer were treated prior to TCM syndromedifferentiation, cross-resistance gas into phlegm, blood stasis within the knot, loss of heat inthe Jin, Qi Sun Micro-IV, and surgical resection of cancer tissue obtained during routine saveand quickly sent to a laboratory.②were taken4different syndromes in patients with esophageal tumor tissue cultured in vitro, non-polluting prepared single cell suspension,divided into experimental and control group, the use of MTT was used to determineesophageal cancer cells in patients with esophageal cancer syndromes5kinds of commonlyused clinical chemotherapeutic drug sensitivity in vitro to calculate the esophageal cancer cellwith different syndromes of various chemotherapeutic agents on the inhibition rate.③datax±s, data were analyzed by test, pairwise comparison between groups using t test to (P <0.05)was considered statistically significant, the data are handled with the statistical packageSPSS13.0.Results:①5kinds of anticancer drugs in60patients with esophageal cancer esophagealcancer cells in vitro drug sensitivity was no significant difference (P>0.05).②5chemotherapeuticdrugs inhibit the rate of cross-resistance group in the gas sputum, phlegm group, loss of heat inthe Jin group displayed moderate sensitivity in the Qi and Yin Deficiency group showed lowsensitivity.③The syndrome of5between the sensitivity of chemotherapy was no significantdifference(P>0.05).④different than between any two syndrome: cross-resistance groupsputum gas compared with the sensitivity of Qiyinliangxu significant difference (P<0.05),phlegm and blood stasis group compared with the susceptibility Qiyinliangxu significantdifference (P<0.05), more than any two of the syndrome showed no significant difference(P>0.05).Conclusion:①esophageal cancer in patients with syndrome differentiation type andsensitivity to chemotherapeutic drugs in vitro correlation,5air delivery of chemotherapeuticdrugs on sputum in patients with esophageal cancer hindered the most sensitive, phlegm andblood stasis type, followed by Tianjin loss hot junction, Qi Yin deficiency type the worst typeof syndrome differentiation of esophageal cancer can help determine the sensitivity ofchemotherapy.②5kinds of anticancer drugs in60cases of esophageal cancer cells in patientswith no significant differences in sensitivity, suggesting that clinical chemotherapy selectedaccording to individual differences in patients with chemotherapy reasonable.③IntegrativeMedicine individualized treatment of esophageal cancer is to sensitivity test results accordingto the specific combination of diagnosis and treatment in patients with treatment programsdesigned to develop the best therapeutic approach.