The Impact Of Acupuncture On Ischemic Brain Injury Model In Rats, TNF-α, Caspase-3Expression

Objective:Observe the effect of acupuncture Du meridian people, Bai Hui, the Ren Zhong, the Da Zhui of Resistance to injury and protection of the ischemic area. Observed ischemic brain tissue TNF-α and Caspase-3expression to explore acupuncture Du meridian people, Bai Hui, the Ren Zhong, the Da Zhui on the brain to produce the protective effect and possible mechanisms for this protective effect。Methods:SD male rats32were randomly divided into model group, sham operation group, DuiZhaoDian group and acupuncture group of four groups and each group of each only8.The establishment of a MCAO rat model, MCAO rat model of vital signs were stable after ZeaLonga score neurological deficit scores to verify the success of the model or not, six treatment of neurological function after the line with HE staining in rat brain tissue pathological changes in the flavor end labeling (TUNEL) assay detection of neuronal apoptosis, and apply the SP immunohistochemistry was detected in rat focal cerebral ischemia in brain tissue TNF-alpha and caspase-3positive cells. Result:1.Nerve functional score:(1) group compared with sham operation group, model group, DuiZhaoDian group, acupuncture group of neural function defect scale increased significantly, difference is very significant meaning (P<0.01);(2)group compared with model, DuiZhaoDian group, acupuncture group of neural function defect score lower, and the meridian group significantly different meaning (P<0.01);DuiZhaoDian group is significant difference(P<0.05);(3)group compared with DuiZhaoDian, acupuncture group of neural function defect scale reduced significantly, and significant difference(P<0.05).2.Epidemic photochemical staining study dyeing:TNF alpha immune response results show that the number of positive cells group compared with sham operation group, model group DuiZhaoDian group of TNF alpha positive points cell count was higher, meaning with significant difference (P<0.01); Group compared with model, DuiZhaoDian group of TNF alpha positive points lower cell count, the difference was statistically significant (P<0.05), and the meridian group of TNF alpha positive cell count, reduce the significant difference (P<0.01); And DuiZhaoDian group compared, and the meridian group of TNF alpha positive cell count, reduce, the difference was statistically significant (P<0.05)3.Image analysis ischemia side caspase-3immune response brain cell count and positive results:group compared with sham operation group, model group DuiZhaoDian group caspase-3points group was higher number of positive cells, meaning with significant difference (P<0.01); Group compared with model, and the meridian of the group Caspase-3positive cell count, reduce, the difference was statistically significant (P<0.01), DuiZhaoDian group of Caspase-3positive cells reduce the number is not obvious, meaningless differences (P>0.05); And DuiZhaoDian group compared, and the meridian group Caspase-three positive cell count, reduced significantly, and there is a significant difference of statistical significance (P<0.01).Conclusion:1. Ischemic damage rats obvious neural function defect signs, ischemia area nerve cells exist cell apoptosis, and acupuncture Du meridian people, Bai Hui, the Ren Zhong, the Da Zhui acupuncture point can obviously improve the rat nerve function and effectively restrain brain ischemia cell apoptosis happened after.2. Acupuncture du meridian people, the Bai Hui, the Ren Zhong, the Da Zhui acupuncture point by inhibiting the neural cells inflammation factors TNF alpha and cascade reaction of the apoptotic protease expression of Caspase-3, delaying the ischemic injury the death of neurons, play to ischemic brain damage of protection.