Preparation And Study Of Personalized Artificial The Sternum Composite Materials

BackgroundThe sternum is a flat bone located in the middle of the chest wall，The shape ofdagger，Sternal manubrium, sternum, xiphoid. Sternum on both sides of ribsconnected to constitute the thorax, since an important role in the protection of themediastinum and chest visceral. If the sternum lesions occur, such as: congenitalmalformations, dysplasia, refractory sternal osteomyelitis, traumatic sternalcomminuted, primary, secondary sternal tumors, The traditional conservativetreatment, the treatment effect is not satisfactory, poor prognosis, there are moreproblems and complications, The study found that patients treated by surgery toobtain a significant effect. Artificial materials for better bone support of the thorax,many artificial materials try to be applied to the chest wall reconstruction, but so farhave not found a very good material. At present, the materials used in the clinicalhave problems, such as binding capacity and organization, organic combination ofhard, loose, shift, activities, easily lead to long-term implantation of the hematoma,fistula, pain, vascular damage and lead to infection and other complications.The emergence of new bio-composite materials, providing a broader range, asartificial material, It has better compatibility than traditional materials, appropriatedegradation rate shaping. The clinical application of tissue engineering Techniqueshave achieved good results in the regeneration, including bone, cartilage, skin andother tissues. Feasible methods and techniques，combined with two, get a newartificial materials and repair methods, And the materials and methods to a series ofstudies of the feasibility as a sternum injury and repair.Research purposes（一）Screening of alternative artificial materials, choose to meet the physical andchemical properties, the organization has good compatibility, preparation of apersonalized repair material composite technology through new materials. （二）The preparation of materials, bio-safety and tissue compatibility evaluation,provide the experimental basis for clinical use.（三）Use of tissue engineering principles and techniques, by observing the cultureof bone marrow mesenchymal stem cells and repair materials, explore the feasibilityof personalized repair material for sternal repair.Research Contents and Methods（一） Design, material selection and preparation of a personalized artificialsternum materialsContrast through a detailed investigation，Polycaprolactone (PCL) as thesternal repair material substrate material，natural bone meal as a nutritionalmaterial; Into personalized porous structure of scaffolds were prepared by meltblending. Materials must meet the mechanical strength to stabilize the thorax, andcertain raw materials and nutrients for tissue repair.（二） Biological Evaluation of a personalized artificial sternum materialsBiological evaluation of medical devices standard ISO10993-1998andGB/T16886-2003standards and requirements，biological safety and tissuecompatibility test of the new bio-composite materials, Including cell toxicity test,acute systemic toxicity test, hemolytic test, the heat source test. The aim is todetermine whether the material has the feasibility of clinical application.（三）Preliminary study of a tissue-engineered artificial sternumThe dog bone marrow mesenchymal stem cell isolation, cultured in a cellincubator, passaged，Personalized porous sternal repair materials and then invitro cultivation of the natural bone meal preparation in the PCL, observing thegrowth of stem cells, proliferation, Provide a theoretical basis and technicalsupport for further research and animal studies of tissue-engineered artificialsternum. The results（一）Add natural bone meal as a nutritional materials in pure PCL，maintainingthe advantages of pure PCL, and Improving the mechanical strength of the composites.The porous structure greatly reduces material degradation, also increased the surfacearea of the material, is more conducive to the organization’s growth recovery.Materials have more advantages than traditional materials.（二）Pure PCL and added different proportions of natural bone meal PCL porouscomposite has better bio-security，No significant cytotoxicity of each componentmaterial, cell morphology, growth, metabolism and proliferation abnormalities,material extracts did not cause a hemolytic reaction and acute systemic toxicity, noobvious heat source. A composite material, the low proportion of natural bone mealno obvious mechanical enhancement, you must reach a certain proportion in order tosignificantly improve the mechanical strength.(三)Dog bone marrow mesenchymal stem cells in the in vitro growth, In the processof co-cultured with the scaffold material, the cells attached to the scaffold material,showing a good biocompatibility, the growth of proliferative, that scaffolds and cellshave good synergy. By comparing the materials in the natural bone meal can beconducive to cell growth, proliferation, differentiation and osteogenesis.Experimental conclusionsPCL/natural bone meal preparation personalized porous composite has goodbiocompatibility and suitable mechanical strength and toughness, can provideeffective support on the thoracic, Order prepared from the3D porous structuresuitable for tissue growth, is an ideal tissue engineered bone materials have broadprospects for clinical use.