Studies On Security, Pharmacology And Efficacy Of Chinese Herbal Medicine Qianshiyang

Qianshiyang is made up by radix asteagali, rhizome coptidis, radix scutellariae, tea polyphenols, agaricus blazei polysaccharide, etc. More than20kinds of components. It is combined and improved which is according to the animal physiological characteristics and based on anti-cancer chinese herbal medicine compound in people. The research directions were taken by quality control, safety, pharmacological effect, bacteriostasis and antiviral actions. Radix asteagali and rhizome coptidis were carried out qualitative determination in thin layer chromatography. Baicalin and epicatechin were carried out quantitative determination in liquid chromatography. Acute toxicity test, chronic toxicity test, liver protection, kidney protection, antioxidant effect, immunity researches were made in vivo of mouse. The inhibition of salmonella and resistance of PRV and PRRS were made in virto.(1) In the test of quality control of Qianshiyang, radix asteagali and rhizome coptidis were carried out qualitative determination in thin layer chromatography, baicalin and epicatechin were carried out quantitative determination in liquid chromatography. Test results had established a set of detection methods of qualitative and quantitative research on Qianshiyang. Baicalin content was2.0425%, epicatechin content was0.7152%.(2) Through the acute toxicity test and chronic toxicity test, Qianshiyang has a characteristic of low toxicity and safety range. It is the actually non-toxic material. Qianshi yang had a remarkable effect on increasing mice’s weight. Qianshiyang is a new type of Chinese herbal medicine compound that has a high development potential.(3) In the liver protection test, three dose groups(0.15g·kg-1,0.3g·kg-1,0.16g·kg-1) were setted. The results showed that the groups of0.3g·kg-1and0.6g·kg-1had significant liver protection. In the kindey protection test and immunity test, three dose groups (0.3g·kg-1,1.5g·kg-1,3g·kg-1) were setted. The results showed that group of0.3g·kg-1had significant effects on protecting kindey and increasing immunity. In the antioxidation test, three dose groups (0.3g·kg-1,0.6g·kg-1,1.2g·kg-1) were setted. The results showed that the three dose groups all had significant antioxidant effect. This research indicated that using ideal dose of Qianshiyang was between0.3g·kg-1and1.2g·kg-1.(4) The test of salmonella inhibition was made by maikangkai medium. The results showed that concentration of Qianshiyang extract was0.1g·mL-1, it had the most remarkable bacteriostasis. And bacteriostasis had a certain relevance to the extrat’s concentration. The test of Qianshiyang resist PRV and PRRS were made by PK-15and Marc-15cells. The results showed that virus were killed in37°C keeping1h. That was the dose of0.004g?mL-1Qianshiyang extracts had the better role of resisting PRV virus, the dose of0.002g?mL-1Qianshiyang extract had the better role of resisting PRS virus. When less than the concentration, the extract had no antiviral action or lightly antiviral effect.