Preparation Technology Study On Micronization Of Ginkgo Biloba Extract

Preparation of Ginkgo biloba extracts (GBE) has treatment of coronary heart disease, hyperlipidemia, brain embolism, agedness dementia, angina et al. Due to low solubility of GBE, some solubilizers usually were added in the preparation of GBE in order to improve its solubility.In the study, in order to enhance the GBE solubility, two methods on micronization of Ginkgo biloba extracts by supercritical antisolvent (SAS) and by spray dryness (SD) were developed. For obtaining the optimum SAS preparation technology of GBE, the effects of four factors, namely, precipitation temperature, pressure, flow rate and concentration of GBE solution on particles diameters were investigated by orthogonal array design. For obtaining the optimum SD preparation technology of GBE, the effects of three factors, namely, entrance hot air temperature, concentration of GBE solution and flow rate on particles diameters were investigated by orthogonal array design. The morphology of the unprocessed and processed GBE particles by SAS and SD was observed using scanning electron microscopy (SEM). Terpene lactones and flavonoids in GBE were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS) and high performance liquid chromatography (HPLC), respectively. In addition, the solubility in unprocessed and processed GBE was tested and the prevention effect of micronized powder by SD method on hyperlipidemia in mice was also discussed.The optimum SAS micronization conditions were determined to be as follows:a precipitation temperature of65℃, a precipitation pressure of30MPa, a GBE solution flow rate of6.0ml/min and a GBE solution concentration of2.5g/L. Under these optimum conditions, micronized GBE with amean particle size (MPS) of81.2nm was obtained the micronized GBE particles were spherical. The optimum SD micronization conditions were determined to be as follows:an entrance hot air temperature160℃, a GBE solution concentration1.25g/L, a GBE solution flow rate of20mL/min. Under these optimum conditions, micronized GBE with a mean particle size (MPS) of1.80μm was obtained and the micronized GBE particles were spherical. Compared with the unprocessed GBE, the contents of terpene lactones and flavonoids in processed GBE by SAS and SD were not significantly different (P<0.05). Finally, the dissolution rates of processed by SAS and SD and unprocessed GBE were investigated and the results proved that there is a significant increase in dissolution rate for processed GBE using the SAS and SD process compared to unprocessed GBE.Compared with the unprocessed GBE, micronized GBE prepared by SD had an obvious advantage in lowing the contents of total cholesterin (TG) and triglycerides (TG) in high-fat diet mice.