| Guizhi Fuling Capsule is a traditional Chinese Medicine Preparation, which is made up of Ramulus Cinnamomi, Cortex MouTan, Persicae Semen, Radix Paeuniae Rubra and Poria cocos. It is traditionally used to treat various kinds of gynecological disease.As a primary ingredient of Guizhi Fuling Capsule, Poria cocos is the sclerotiorum of Poria cocos(Schw.)Wolf.(Polyporaceae), which is traditionally used in Chinese herbal prescriptions as diuretic, sedative and tonic. It is mainly distributed in Yunnan, Anhui and Hubei provinces of China, we do the bioactive screening researches on its crude extract by the high-throught model for TNF-a production and model of Hela cytotoxicity. The experiments showed that the ethylacetate and alcohol extract had activities.The95%ethanol extract of the Poria cocos as well as the alcohol and aqueous extract of Guizhi Fuling capsule were investigated respectively on their chemical constituents systematically by various kinds of chromatographic methods. The isolates were elucidated on the basis of spectroscopic analysis and chemical methods as well. Among the forty-one compounds isolated from poria cocos, the compound13is a new compound, the compounds (18,21-27,30,32,34-36,39-40) are isolated from the poria cocos for the first time. These compounds are listed as follows:pachymic acid (1), eburicoic acid (2), tumulosic acid (3), trametenolic acid (4), dehydropachymic acid (5), dehydrotumulosic acid (6), dehydroeburicoic acid (7), dehydrotrametenolic acid (8),3-epi-dehydropachymic acid (9),3-epi-dehydrotumulosic acid (10), polyporenic acid C (11),6a-hydroxy-polyporenic acid C (12),6a-hydroxy-dehydrotumulosic acid (13),3β-acetyl-16a-hydroxy-dehydrotrametenolic acid (14), pachymic acid methyl ester (15), palmitic acid (16), oleanic acid (17), carboceric acid (18), ergosta-5,7,22-trien-3β-ol (19), ergosta-6,22-diene-5,8-epidioxy-3-ol (20),3,5-dihydroxy-ergosta-7,22-dien-6-one (21),3β,5a,9a-trihydroxy-ergosta-7,22-diene-6-one (22), ergosta-7,22-diene-3-one (23),6,9-epoxy-ergosta-7,22-diene-3-ol (24), ergosta-4,22-diene-3-one (25),3β,5α,6β-trihydroxy-ergosta-7,22-diene (26), ergosta-5,6-epoxy-7,22-dien-3-ol (27), ergosta-4,6,8(14),22-tetraen-3-one (28),β-amyrin acetate (29), α-amyrin acetate (30), daucosterol (31),β-sitosterol (32), adenosine (33), ribitol (34), mannitol (35), oleanic acid3-o-acetate (36), a-D-glucose (37), a-D-galactose (38), trehalose (39), galactitol (40), histidine (41). Seven-teen compounds, including nine compounds that had been isolated from the Poria Cocos were obtained from alcohol and aqueous extract of Guizhi Fuling capsule. Thet were identified as follows:oypaeoniflora (42), caffeic acid (43), mudanpioside F (44), guanosine (45), alanine (46), leucine (47), proline (48), arginine (49).Part of compounds obtained from Poria cocos displayed moderate inhibitory activity against seven human tumor cells (A549, Bel-7402, BGC-823, HCT-8, A-2780, KB, MCF) by MTT method.Moreover, a HPLC method has been developed for simultaneously determination of four constituents, dehydropachymic acid, dehydrotumulosic acid,3-epi-dehydropac-hymic acid, polyporenic acid C. The analysis was achieved on a C18column (Pravil,4.6×250mm,5μm) using a mobile phase of a mixture of acetonitrile and0.2%formic acid in a total run time of90min and a diode array detector was set at242nm. The flow rate was1ml·min-1. The average recoveries(n=6) were96.28%,101.00%,98.50%and99.6%for dehydrotumulosic acid, polyporenic acid C,3-epi-dehydropachymic acid and dehydropachymic acid respectively. The relative standard deviations were1.2%,0.2%,0.2%,0.6%respectively. The method is sample, sensitive, reliable and reproducible which can be used for the quality study of Guizhi Fuling capsule. |
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