Studies On Chemical Constituents Of The Melicope Ptelefolia And The Determination Of Effective Component In Other Two Medicinal Plants

Objective: The Melicope ptelefolia (ChamP.exBenth.) Hartley.belongs to Melicopegenus of Rutaceae. As a traditional medicinal plant, it was commonly used by people lived insouth of China. There were effective in clearing heat and removing toxicity, reducing swellingand ease pain. It can be used to treat such as jaundice, eczema, dermatitis, sore throat,rheumatism, malaria, traumatic injuries, bites and so on. In this paper, the chemicalcomponents of the Melicope is related and analyzed, so did the plant Melicope ptelefolia(ChamP.exBenth.) Hartley.Then some chemical components was isolated and puried fromit’s stems, and the compounds were identified by physical and chemical properties, the basisof spectral data and reference. Last, the effective component in two medicinal plants-Microcos Paniculata L’s and Manglietia Hainanensis’s were determined by UltravioletSpectrophotometric methods, and putting forward the active ingredient for the determinationof methods and quality control standards and so on.Method:(1).The75%EtOH extract of Melicope ptelefolia (ChamP.ex Benth.) Hartleywas re-extracted with petroleum ether, chloroform, ethyl acetate, and n-butanol successivelyto give four fractions, the crude extract and every fraction screened by Jurkat cell model. Thechemical constituents were isolated by means of silica gel column chromatography (CC),Sephadex LH-20, preparative thin layer chromatography (PTLC) and HPLC etc. Then variousspectroscopic methods including spectrum analysis methods were used to elucidate thestructures.(2). Under the experimental conditions including ursolic acid as the reference,5%vanillin-acetic acid and perchloric acid as color developer, the content of total triterpenoidicacid in the leaves and stems of Microcos Paniculata L and Manglietia hainanensis weredetermined by ultraviolet spectrophotometric method. Under the experimental conditionsincluding genistein as the standard，the content of the isoflavones in the leaves and stems ofMicrocos Paniculata L and Manglietia hainanensis were determined by ultravioletspectrophotometric method also.Result: Twelve compounds were identified by the modern spectrum technology, unifiesphysicochemical method and literature comparison as phthalic acid dibutyl ester (1)， vanillin(2)，β-sitosterol(3)，friedelin(4)，β-daucosterol(5)，leptonol(6)，phthalic acid butylisobutyl ester (7)，methylleptol A(8)，isoevodionol(9)，lepten A(10)，evodione(11)，(scopoletin)(12). And twelve compounds were obtained from the stems of Melicopeptelefolia (ChamP.exBenth.) Hartley. Compound(2)、(4)、(7)、(12) were isolated fromMelicope ptelefolia (ChamP.exBenth.) Hartley. for the first time.The content of the total triterpenoidic acid in the stems and leaves of Microcos Paniculata L was1.09%and2.77%; in the Manglietia hainanensis was0.47%and1.94%. The content of the totalisoflavones in the stems and leaves of Microcos Paniculata L was0.07%and0.23%.; in theManglietia hainanensis was0.078%and0.24%.The ultraviolet spectrophotometric method operatedconvenientely，the results was stable，and has good repeatability.