Effect Of Meloxicam On Cums-induced Depressive Behavior In Rats

Aim: To investigate the effect of meloxicam on the depressive-likebehaviors caused by chronic unpredictable mild stress （CUMS） in rats.Methods:（1） Male SD rats were randomly divided into following groups: controlgroup, CUMS group, sertraline group, meloxicam1,3and5mg·kg-1groups.Chronic unpredicted mild stress was taken to establish rat depression model.Sertraline (5mg·kg^-1·d) and meloxicam (1,3and5mg·kg^-1·d) wasadministrated after stress procedure for20days.（2） The open-field test, elevated plus-maze （EPM） assay and forcedswimming test were used to evaluate the depression behaviors of rats. HEstaining was used to observe hippocampal pathological changes. SYP,BDNF, PSD-95and5-HT1Areceptor protein expression was detected byimmunohistochemistry. MDA concentrations and SOD activity wereanalyzed by method of biochemistry, IL-6, IL-1β and TNF-α levels weredetected by method of ELISA.Results: As compared with that of the control group, administration ofstress caused a decrease of the crossing and rearing movement times from open-field test, a significant increase of closed-arms retention time fromelevated plus-maze and immobility from forced swimming test. The levelsof MDA, IL-6, IL-1β and TNF-α of rats significantly increased, and SODactivity significantly decreased in model group compared with control group.Hippocampal neurons in model rats showed obviously loss andkaryopycnosis, CUMS significantly decreased the expression of SYP, BDNF,5-HT1Areceptor, and PSD-95protein in rat hippocampi.Meloxicam remarkably improve depressive behavior and prevented thehippocampal neurons from karyopycnosis induced by CUMS. Meloxicamsignificantly increased the expression of SYP, BDNF,5-HT1Areceptor andPSD-95protein in CUMS rats. The change of MDA, IL-6, IL-1β and TNF-αconcentrations and SOD activity was significantly blunted in CUMS rats bymeloxicam.Conclusions: Meloxicam have a potential therapeutic benefit againstCUMS-induced depression and antioxidative stress and anti-inflammationmay be involved in its protective mechanism.