Study On The Relationship Of Interleukin-10-592C/A Polymorphism And Premature CHD

ObjectiveCompare the distributive regularity of the IL-10-592site genetype betweenpremature CHD group and healthy group, age≤45years group who is from thepremature CHD group and healthy group, to explore the relationship between-592C/A polymorphism and premature CHD.Methods118premature CHD patients confirmed by CAG were chosen from SecondAffiliated Hospital of University of South China, with the average age of46.42±4.74years old, including86cases of acute coronary syndrome(ACS),32cases of stableangina pectoris(SAP). For a futher study, selected56cases whose age≤45years, andthey were from the118cases of the premature CHD. The diagnostic standard was the2007ACC/AHA guidelines. Other cardiovascular diseases, hepatic, renal, endocrineand inflammatory diseases were excluded. The average age of124cases healthycontrol group is47.54±4.56years old. IL-10-592C/A polymorphisms were analyzedusing restriction fragment length polymorphism (RFLP) and agarose gelelectrophoresis methods in both CHD and control groups. Genotype distributions andallelic genes of the polymorphisms between CHD patients and controls were assessedby Chi-square analysis.Results1. Distribution of IL-10-592different genotype of the two groups showed nosignificant difference by the Hardy-Weinberg equilibrium (P>0.05), the sample couldrepresent the group.2. Statistics showed no significant difference when the studies were based on thedifferentiation of age, gender, fasting blood-glucose, weight, SBP, DBP, LDL and HDL (p>0.05); difference was significant when the studies were based on smokingratio, family history of cardiovascular disease, TG and TC(P <0.05).3. Study on the premature CHD and healthy groups showed: Among the118premature CHD patients, there are8cases of AA,40cases of AC70cases of CC, thegenetype frequences were6.78%,33.9%and59.32%, respectively, the ratio of the Aallele is23.73%, and the ratio of the C allele is76.27%. Among the124controlgroups,9cases of AA,33cases of AC,82cases of CC, the genetype frequences were7.26%,26.61%and66.13%, respectively, the ratio of the A allele is20.56%, and theratio of the C allele is79.44%. IL-10-592genotypic distributions and the frequenciesof alleles showed no significant difference(x2were1.53,0.703, P>0.05). However,among the56cases with ages≤45years, including7cases of AA,35cases of AC,14cases of CC, genetype frequences were12.5%,62.5%and25.0%, respectively, theratio of the A allele is43.75%, and the ratio of the C allele is56.25%. Among the64cases with their age-matched control group, including3cases of AA,12cases of AC,49cases of CC, the genetype frequences were4.69%,18.75%and76.56%,respectively, the ratio of the A allele is14.06%, and the ratio of the C allele is85.94%.The genotypic distributions and the frequencies of alleles of the two groups hadsignificant differences (x2were33.46,26.162, P <0.05). The frequencies of AAgenotype and alleles were much higher in premature CHD group(age≤45years) thanthe control group. Compared with those who carry CC genes, AA genes can increasethe risk of catching CHD to8.167times(OR:8.167;95%CI:1.846-35.775), and Aallele to4.753times(OR:4.753;95%CI:2.55-8.859).4. Study on the ACS and SAP group showed: Among86patients of ACS groups,there are6cases of AA,27cases of AC,53cases of CC, the genetype frequenceswere6.98%,31.39%and61.63%, respectively, the ratio of the A allele is22.67%,and the ratio of the C allele is77.33%. Among32patients of SAP groups,2cases ofAA,13cases of AC,17cases of CC, the genetype frequences were6.25%,40.63%and53.12%, respectively, the ratio of the A allele is26.56%, and the ratio of the Callele is73.43%. IL-10-592genotypic distributions and the frequencies of alleles showed no significant difference (x2were0.874,0.39, P>0.05).5. Among the81cases of males in premature CHD, including6cases of AA,23cases of AC,52cases of CC, the genetype frequences were7.41%,28.39%and64.2%, respectively, the ratio of the A allele is21.6%, and the ratio of the C allele is78.4%. Among the37cases of females, including2cases of AA,17cases of AC,18cases of CC, the genetype frequences were5.4%,45.95%and48.65%, respectively,the ratio of the A allele is28.38%, and the ratio of the C allele is71.62%. IL-10-592genotypic distributions and the frequencies of A and C alleles showed no significantdifference between different sex in premature CHD group(x2were3.421,1.288,P>0.05). Among the82cases of males in control group, including7cases of AA,19cases of AC,56cases of CC, and genetype frequences were8.54%,23.17%and68.29%, respectively, the ratio of the A allele is20.12%, and the ratio of the C alleleis79.88%. Among the42cases of females, including2cases of AA,14cases of AC,26cases of CC, the genetype frequences were4.77%,33.33%and61.9%,respectively, the ratio of the A allele is21.43%, and the ratio of the C allele is78.57%.IL-10-592genotypic distributions and the frequencies of A and C alleles showedsignificant difference between different sex in control group(x2were1.8,0.058,P>0.05).Conclusion1. The age≤45years of the people, the C/A polymorphism of IL-10-592isassociated with premature CHD; AA genotype and A allele of IL-10-592is possiblyone of the susceptibility genes of CHD.2. IL-10-592genotypic distributions and the frequencies of alleles show nodifference between ACS group and SAP group.