Effects Of Follicle-stimulating Hormone On Postmenopausal Osteoporosis

Osteoporosis is a common disease for postmenopausal women.Its mechanism is not absolutely clear.Recently,the relationship between FSH and postmenopausal osteoporosis was observed.In this research, we studied the relationship between FSH and postmenopausal osteoporosis,and observed the effects of FSH on osteoclast phenotypic and functional genes.Experiment One:Changes of serum hormones and BMD in postmenopausal womenObjective:To observe the changes of FSH and BMD in postmenopausal women,and research the relationship between FSH and postmenopausal osteoporosis.Methods:We randomly chose248postmenopausal women from Jinan,whose ages are older than5O.And we excluded the persons with diseases connect with bone metabolism or take long-term drugs affecting bone metabolism.RIA was used to detect serum FSH、 LH、E2,and dual energy X-ray absortiometry was used to measure forearm BMD.Then we analyzed the changes of serum FSH、LH、E2with age. And serum FSH between postmenopausal osteoporosis group and control group was compared.Results:1.With age growing, serum FS、LH raised,and peak at about60,then serum FSH、 LH reduced as older.Serum E2declined as age growing.And BMD decreased in postmenopausal women.2. Serum FSH was negatively correlated with BMD in postmenopausal women, serum FSH in postmenopausal osteoporosis women increased notablely compared with non-osteoporosis women. Conclusions: This study revealed the changes of FSH、LH、E2and BMD as ages growing suggested that the circulation concentration of FSH may play an important role in the acceleration of bone loss in postmenopausal women.Experiment Two: Effects of FSH on osteoclast phenotypic and functional genes Objective:To observe the effects of FSH on osteoclasts differentiation from RAW264.7cells,and genes expression related to osteoclasts differentiation and function.Methods:Osteoclasts were induced by50ng/ml RANKL from RAW264.7cells in vitro,and treated with0ng/ml、5ng/ml、10ng/ml、20ng/ml FSH, after osteoclsts maturated,TRAP staining was used to identify osteoclsts,and mRNA expression of osteoclastic phenotypes and functioning genes as RANK、TRAP、MMP-9、Cathepsin K in different groups were detected by Real-time PCR.Results:RAW264.7cells could differentiate to mature osteoclasts in vitro by RANKL,and Real-time PCR demonstrated,treated with different concentration FSH,compared with control group,the mRNA expression of osteoclastic phenotypes and functioning genes as RANK、TRAP、MMP-9and Cathepsin K increased,and the change of mRNA expression were dose related.Conclusions:1.As a superior inducer,RANKL could promote RAW264.7cells differentiate to mature osteoclasts.2.FSH improved the mRNA expression of osteoclastic phenotypes and functioning genes as RANK、TRAP、MMP-9、Cathepsin K, and the change of mRNA expression were dose related.