The Study On The Significance Of IL-17and TGF-β1in Oral Lichen Planus And Oral Squamous Cell Carcinoma

PurposeOral Lichen Planus (OLP) is a common oral mucosal chronic inflammatory disease in dental clinic. Its morbidity rate is about0.51%and the patients often suffer relapses. It can be healed but then occurred again repeatedly. OLP has been classified as one possible precancerous state by WHO. The cause and morbidity of it are related to many reasons. Lots of research proved that immune adjustment anomaly mediated by T lymphocytes plays a major role in the process of development of the disease. Oral squamous cell carcinoma is the most common malignant tumor in oral and maxillofacial region. It makes up about above80%, and the body’s immunological function is closely related to its origination and development. When the body’s immunological function is low or restricted, the morbidity rate of OSCC increases. While during the insidious progressive growth of tumor, the patient’s immunological function is inhibited and the two sides interact as both cause and effect. The growth and decline of respective factors play an important role in the development of tumor.Thl7cell is one category of CD4+effectors’lymphocyte subsets that is found in recent years and it is characterized by producing IL-17(interleukin-17, IL-17) which play an important role in host defense and immnoregulation. As a strong pro inflammatory cytokine, IL-17can induct other cytokines and chemo tactic factors to express, so that it will mediate the inflammatory corpuscles’recruitment, infiltration and tissue damage. Transforming Growth Factor Betal(TGF-β1), is a kind of multi-functional cell factors, participating in many biological effects, such as cell differentiation proliferation, immunoregulation, cell apoptosis, cancer and metastasis. Research found TGF-β1take part in the illness and cancerous process of OLP. This experiment tested the expressions of IL-17, TGF-β1in oral lichen planus and oral squamous cell carcinoma by immunohistochemical techniques, discussed the possible function and mechanism of IL-17, TGF-β1in OLP and oral squamous cell carcinoma. This paper provided new thought in the etiology, clinical remedy and preventive treatment in OLP and OSCCMethodExclude other systematic diseases. Choose30cases of OLP diagnosed via clinical and pathological research, without any systematic treatments in3months. The20cases of oral squamous cell carcinoma, Dexcluding other systematic diseases, don’t go through radiation and chemotherapy before operation. Use Immunohistochemical techniques to test the expression of IL-17and TFG-β1in OLP and oral squamous cell carcinoma in contrast to normal mouth mucosa. The acquired data is analyzed by the SPSS15.0statistical software. Results1. IL-17’s expression in OLP and squamous cell carcinoma:IL-17has positive expression in normal oral cavity mucous epithelium layer which distribute mainly in cuticular layer, stratum granulosum and part of stratum spinosum, while a small amount of cells express in the mesenchyme and the positive immunohistochemical stains mainly distribute in cytoplasm; in the OLP tissue, the positive stains of IL-17is less in its epithelium layer and is mainly distribute in the laminae propria sporadically. In highly differentiated squamous cell carcinoma, IL-17expresses strong in the center of cancer nests but it is weak in the periphery and expresses little in the mesenchyme; in moderately differentiated squamous cell carcinoma, the cancer cells are weakly positive and mesenchymal cells express more. Via the analysis of stasistics, IL-17’s expression in OLP and OSCC has a significant difference compared with NOM group (P<0.05), and also a significance difference between OLP group and OSCC group (P<0.05)2. TGF-β1’s expression in OLP and squamous cell carcinoma:TGF-β1has strong positive expression in normal oral cavity mucous membrane layer and inherent layers which mainly distributes the basalis, stratum spinosum and stratum granulosum. Lamina propria has diffuse expression. The tissues in OLP are weak positive which mainly distribute in the whole epithelium layer. Lamina propria has diffuse positive expression, too. There is a significant difference (P<0.05). TGF-β1is positive in tumor cells in9cases of OSCC tissue but expresses little in the mesenchyme. TGF-β1is weak positive in tumor cells in3cases of OSCC tissue but expresses a lot in the mesenchyme. TGF-β1is negative in8cases of OSCC tissue.Conclusion1. Abnormal expression of IL-17and TGF-β1in OLP tissue points out that they are possibly related to the origination and development of the diseases.2. The exceeding expression of IL-17in OSCC tissue possibly plays an important part in the origination and development of the disease.3. The TGF-β1’s expression diversity in OSCC tissue points out that it plays a double-regulated role in the origination and development of the diseases. It is also related to the stage, classification and prognosis of OSCC.