Effect Of Iodine Excess On Oxidative Damage

[Objective]In this study, rats were fed with Iodine excess, the establishment of the rat models of Iodine excess, using of the measuring of the determination of plasma HCY、MDA、SOD and GSH-Px, to observe the effects of different concentrations of Iodine on oxidative damage, and to study the mechanism of the excess Iodine on oxidative damage.[Material and Methods]1. Experimental animals and groups:selecting of approximately1month after weaning, weighing100-120g outbreeding closed colony of Wistar rats (purchased from the Animal Experimental Center, Shandong University, is a SPF/VAF-class animals), according to weight and sex, the rat were randomly divided into4groups, the order as follows:①Normal control group(NI):feed normal diet and drink normal water;②10HI group:drink10multiple excess Iodine water;③50HI group:drink50multiple excess Iodine water;④100HI group:drink100multiple excess Iodine water; To weigh rats’body weights weekly and collect urine to observe their Iodine metabolism at2,4and6months later.N=8.2. Records of body weight data of the rats:The above animals weekly weigh in and record. Feeding for6months.3. Measuring HCY、MDA、SOD and GSH-Px of Rats in each group, Data were analyzed with normal ANOVA using the SPSS software(SPSS, version16.0).[Results]1. Compared with NC, homocysteine and malondialdehyde increased in50HI group and100HI group (p<0.01). 2. Compared with NC, SOD and GSH-Px decreased in50HI group and100HI group (p<0.01).[Conclusions]Moderate iodine excess does not have any effect on homocysteine and malondialdehyde of serum in rats, but with the increasing of iodine concentration, homocysteine and malondialdehyde rise in rats, at the same time, SOD and GSH-Px decreased in rats.