The Study On Acanthopanax Improving Hippocampus Cholinergic Nerve System Mechanism Of Learning And Memory Impairment Rats

Objective:Research Acanthopanax improve scopolamine-induced learni-ng the cognitive ability of memory impairment in rats and the mechanism of hippocampus cholinergic nervous system.Methods:Wistar rats as experimental subjects:â‘ learning detected by the hexagonal maze experimental setup of the behavior in rats;â‘¡using enzyme-linked immunosorbent assay for the determination of Ach, MAChR, using colorimetric determination of AchE activity, with electron microscopy technique to observe changes of the Rat Hippocampal CA3ultra structure, exploring the impact of cognitive ability and cholinergic nervous system that Acanthopanax on scopolamine-induced memory impairment in rats.Results:(1)Acanthopanax on memory impairment in rats learning and memory ability affect the experimental results show that:Compared with the control group, model group finding time,finding frequency and cognitive rate reduced(p<0.01);Compared with model group, the Acanthopanax group and the Huperzine A group finding time reduced(P<0.01) and the cognitive rate increased(p<0.01), Huperzine A group finding frequency increased (p<0.05),the Acanthopanax group finding frequency had the increased trend but no significant difference.(2)Acanthopanax on the impact of memory impairment in hippocampus of rats Ach content results show that:Compared with the control group, Ach content of the Acanthopanax group and Huperzine A group increased significantly (p<0.01), the Ach of model group had the induced trend, but there was no significant difference; Compared with the model group, Ach content of Acanthopanax group and Huperzine A group increased significantly (p<0.01). (3)Acanthopanax on the impact of memory impairment in hippocampus of rats AchE activity results show that:Compared with the control group, the AchE activity of Huperzine A group induced (p<0.01), Acanthopanax induced but had no significant difference; Compared with the model group, the AchE activity of Huperzine A group induced (p<0.01), Acanthopanax induced but no significant difference.(4)Acanthopanax on the impact of memory impairment in hippocampus of rats M-AChR content results show that:Compared with the control group, M-AChR content of the Acanthopanax group increased(p<0.05), model group induced(p<0.05); Compared with the model group, M-AChR content of the Acanthopanax group increased(p<0.05), Huperzine A group increased but no significant difference.(5)Acanthopanax on the impact of memory impairment in rat hippocampal CA3region of the ultra structure results show that:â‘ Changes in capillary ultra structure of the hippocampal CA3region: Compared with the control group, the capillaries of model group depressed deformation significantly, basement membrane is not clear; Compared with the model group, the capillaries of Acanthopanax group is mellow and full, basement membrane is clear, the capillaries of Huperzine A group depressed slightly.â‘¡Changes in neurons ultra structure of the hippocampal CA3region: Compared with the control group, hippocampal neurons nuclear membrane of the model group is shrinking slightly; Compared with the model group, hippocampal neurons nuclear membrane of the Acanthopanax group and Huperzine A group are smooth.â‘¢Changes in synaptic ultra structure of the hippocampal CA3region: Compared with the control group, the number of synaptic vesicles reduced, synaptic cleft stage widened, thickness of postsynaptic membrane dense material reduced in the model group; Compared with the model group, the number of synaptic vesicles increased, synaptic cleft narrowed down, thickness of postsynaptic membrane dense material increased relatively.Conclusion:(1)Acanthopanax can improve the learning and memory activity of rats that scopolamine-induced learning and memory impairment.(2)The Mechanism Acanthopanax Improving Cognitive Ability and hipppcampus Cholinergic nerve system of Rats is by reducing the Ach activity, increasing the M-AchR content of postsynaptic membrane, protecting structure of capillaries and synapses of the hippocampus neurons, and reducing the metabolism and transformation of Ach, increasing the opportunity to bind receptor, increasing its secretion in the synapse.