Effects Of ω-3Polyunsaturated Fatty Acids On Liver Function And Inflammatory Response In Burned Rats

Objective:To investigate the effects of ω-3polyunsaturated fatty acids on liver injuryand inflammatory responses after severe burn.Methods:A total of72SD rats were randomly divided into3groups: the normal controlgroup (A group,n=8); the experimental group (B group,n=32); the treatment group (Cgroup,n=32).A group rats used warm water to simulate burn process, B group and Cgroup rats used30%total body surface area(TBSA) full thickness burns model. Rats ingroup A were injected NS (2mL/(kg·d))by tail vein, Rats in group B were injected20%middle-long-chain fatty emulsion (1mL/(kg·d)) by tail vein, Rats in group C wereinjected ω-3fish oil (2mL/(kg·d)) by tail vein at the same time for each day. Beforeinjury group (Group A) and after injury groups (Group B&C)12h,1d,4d,7d,10d weremeasured respectively the levels of serum LPS,PGE2, IL-6, TNF-α;detected expressionof NF-κBp65in intestinal tissue by immunohistochemical staining; determinedexpression of liver tissue TNF alpha mRNA by RT-PCR technology and observedmorphological changes of ileum by normal light and TEM. Each subgroup had8rats.Results:1.Serum LPS, PGE2, TNF-α,IL-6were significantly higher than pre-injury ateach time point after injury in B&C,(P <0.01).. The levels in C group were lower thanin B group, and were significantly different in4d,7d,10d after injury (P <0.05or P<0.01).2.Compared with Group A by TEM, Group B after injury nuclear membrane ofthe liver tissue swelling,Mitochondria swelling and free bubble change,Crestbreakthrough bad;Group C after injury nuclear membrane of the liver tissue swelling(compared with group B mitigation) or complete,Swelling of mitochondria (compared with group B mitigation),Crest breakthrough bad (compared with group B mitigation)or complete.3.Compared with Group A by Immunohistochemistry, the expression ofliver tissue NF-κBp65increased in Group B, group C;Compared with group B, theactivity of NF-κBp65was significantly decreased in group C.4.Group B, group C at alltime points after injury the expression of liver tissue TNF-α mRNA was significantlyhigher than group A;Compared with group B, after injury,4d,7d, the expression ofliver tissue TNF-α mRNA significantly reduced in group C.Conclusions:1.ω-3PUFAs reduce the levels of serum LPS, PGE2,TNF-α andIL-6,reduce the expression of liver tissue NF-κBp65,reduce the expression of livertissue TNF-α mRNA.2.ω-3PUFAs reduce the damage of liver tissue after burn.3.Thepossible mechanism of ω-3PUFAs in reducing the expression of liver tissue nucleartranscription factor NF-κB65,block NF-κB signaling pathway, reduce the expression ofTNF-α mRNA, reduce the production of various inflammatory mediators.