Studies On Intervention In Secondary Metabolites In Lonicerajaponka Thunb. And Mahonia Heald (Fort.) Carr. In Response To The Technology Of Ultraviolet Induction

Objective: To investigate the influence on contents of secondary metabolites inpostharvest flower buds of Lonicera japonica Thunb. and leaves of Mahonia bealei (Fort.)Carr. in response to the technology of ultraviolet induction. Induce and culture fresh flowerbuds of Lonicera japonica Thunb. and leaves of Mahonia bealei (Fort.) Carr. usingappropriate intensity of ultraviolet induction to enhance their pharmacological activity byimproving the contents of active ingredients in herbs.Methods: We screened and established the best technical solution of ultravioletinduction, and disposed freshly isolated flower buds of Lonicera japonica Thunb. andleaves of Mahonia bealei (Fort.) Carr. with ultraviolet induction in vitro. And then theblank control group with the induced ones were set in the same samples by culture, drying,crushing, extraction. Fingerprints of the selected objects of our study were established byHPLC-DAD. We found that the contents of some secondary metabolites how to changedue to compare and analysis of the fingerprints and we identified the types of thesecompounds by HPLC-MS or the standard. We investigated the antioxidant power of thetotal methanol extract of honeysuckle by DPPH assay with IC50represents the level ofantioxidant activity.Results: The contents of seven secondary metabolites increased significantly by theuse of ultraviolet induction in vitro to fresh honeysuckle flower buds which are 3,5-di-O-caffeoylquinic acid,4,5-di-O-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid,secologanic acid, secoxyloganin, secologanin and (E)-aldosecologanin respectively. Thefirst three are isochlorogenic acids and after four are iridoid glycosides. Because ofdifferent sensitivity of these seven compounds on UV-A and UV-B radiation, the amplitudeof increase is also different. The results by DPPH assay showed that the antioxidantactivity of the total methanol extract of honeysuckle was significantly enhanced afterultraviolet induction. It matched the results that the contents of two types of compoundswhich had antioxidant activity increased dramaticly. Similarly, the UV-B radiation hadgreat impact on the metabolism of three alkaloids (berberine, jatrorrhizine and palmatine)in leaves of Mahonia bealei (Fort.) Carr. After induction of UV-B, the contents of threealkaloid changed significantly.Conclusion: Ultraviolet induction has remarkable effects on accumulation of caffeoylquinic acids and iridoid glycosides in flower buds of Lonicera japonica Thunb. and threekinds of alkaloids in leaves of Mahonia bealei (Fort.) Carr.