Experimental Study Of HMGB1Expression In Paclitaxel Resistant Ovarian Cancer

Objective:To provide related proteins’information and biological function for the study about the resistance mechanisms of the ovarian cancer paclitaxel. And to explore the correlation and possible mechanisms of HMGB1in the drug resistance of the ovarian cancer paclitaxel, it provided new clues and experimental evidence for the research about the resistance mechanisms of the ovarian cancer paclitaxel.Methods:In the preliminary research, we used the proteomic method to analyze the total protein of the ovarian cancer paclitaxel sensitive cell strains and resistant cell strains, and found24differentially expressed proteins. In this study, first of all, it used the bioinformatics to generalize and integrate the differentially expressed proteins’information, and filtered and predicted the functions of these proteins by analyzing and searching with the software of BinGO. Then it selected the HMGB1(the high protein expression in paclitaxel-resistant ovarian cancer cell lines,) as the target protein, and used the Western blot and immunofluorescence to detect the expression levels and localization of HMGB1protein in paclitaxel-resistant and paclitaxel-sensitive ovarian cancer cells lines.Results:1、We established the molecules network of24differentially expressed proteins and their interacting proteins by using Bioinformatics; BinGO software’s analysis revealed that the24differentially expressed proteins involved in a variety of cellular processes and performed enrichment in the glucose metabolism and regulation of apoptosis; Through the study of the resistance mechanism from the regulation of apoptosis, some important proteins were filtered, which included HMGB1、PHB、ANXA1、HSPB1、SFN、ARHGDIA、SOD2.2、Western-blot results revealed that the HMGB1protein expressed both in SKOV3/Taxol-25and SKOV3, and the expression was higher in SKOV3/Taxol-25, the difference was statistically significant (p<0.05).3、Immunofluorescence results revealed that the HMGB1protein had different levels of expression in SKOV3/Taxol-25and SKOV3, the expression in SKOV3/Taxol-25was significantly higher than in SKOV3, the difference was significant statistically significant (p<0.01); And the expression in the cytoplasm of SKOV3/Taxol-25was also higher than that is in SKOV3, the difference was statistically significant (p<0.01).Conclusions:1、This experiment provided a wealth of biological information for studying the paclitaxel resistance mechanisms of ovarian cancer, and some new clues for filtering related proteins which may be involved in the process of paclitaxel resistance of ovarian cancer.2、The high expression of HMGB1protein may be associated with the occurrence of resistance to paclitaxel in ovarian cancer.3、HMGB1protein may induce the occurrence of resistance to paclitaxel in ovarian cancer through transfer from the nucleus to the cytoplasm and playing its biological effects outside the core.