Expression Of MiR-223and Its Target P2Y12in The Peripheral Blood With Acute Myocardial And Cerebral Infarction

Background:Myocardial infarction and cerebral infarction are main pathological characteristics of cardiovascular and cerebrovascular disease, which seriously threatens to human being healthy and is the main cause of high mortality in patients with clinical emergency. Although clinical manifestations are not the same, the mechanisms of myocardial infarction and cerebral infarction have many similarities. And abnormal platelet function is a common mechanism underlying cardiovascular and cerebrovascular disease. Small ribonucleic acids (miRNAs) are small non-coding RNAs of19-23nucleotides, which can regulate the target gene expression at post-transcriptional level by interacting with complementary sites in the3’untranslated sequences of the target gene. miRNAs are shown to play an important role in biological process and be involved in the pathophysiology of various diseases. The vast majority of miRNAs have their independent gene sequences and regulatory elements, and also a small number located in intergenic regions of the protein coding genes participate in the transcription of protein-coding genes. Recent researches have demonstrated that miR-223is involved in the regulation of platelet function due to its high expression in platelets, and plays a crucial role in the progression of thrombotic cerebrovascular disease.Objective:To explore the potiential significance of miR-223as a novel biomarker for dignosis for acute myocardial and cerebral infraction, we measured the expression of miR-223and P2Y12in peripheral blood mononuclear cells (PBMCs) in patients with acute myocardial and cerebral infraction.Methods:There were three groups of subjects:control group (n=31), acute myocardial infraction group (n=44), acute cerebral infraction group (n=33). The expression of miR-223and P2Y12in PBMCs were measured by real-time PCR.Results:The expression of miR-223, but not its target gene P2Y12, in PBMCs in patients with myocardial infraction were significantly higher than that of the control group; there were no diffrences in the expression of miR-223and P2Y12between control subjects and patients with cerebral infarction. The expression of miR-223was positively correlated with target gene P2Y12expression in all subjects enrolled in this study.Conclusion:The level of miR-223in PBMCs may be a novel biomarker for dignosis for acute myocardial infraction. As for its dignosis significance in acute cerebral infraction needs to be further investigeted in a larger population.