Effects Of Ginsenoside Rh2on Proliferation And Apoptosi In Human Lung Adenocarcinoma Cell A549

Ginseng (Panax ginseng) is Araliaceae perennial herb that contains various bioactive substances, which could prevent tumor, retard aging process, provide radioresistance and many other biological applications. Ginsenoside is the main active ingredient of Panax ginseng. Ginsenoside G-Rh2is a kind of ginseng saponin, which has been approved by a large number of experiments that it could block tumor cell cycle, induce tumor cell apoptosis, reduce the degree of tumor malignancy by inducing the tumor cell differentiation, also can inhibit tumor metastasis; enhance the immune capacity, thereby control the tumor growth. It could also be applied with other anti-cancer drugs to enhance their efficacy. G-Rh2has strong and wide anti-tumor effects. It could produce different effects to different tumor cells with multiple targets and links; the various effects also associate and influence each other. Besides, it is not easy to produce drug resistance and contains low toxicity to normal cells, which make it a heat research topic in recent years. It has achieved a consensus that the signal pathway of NF-KB, Fas/FasL system, caspase family and other protein factors in G-Rh2has positive effects to the apoptosis of A549; however, it’s rarely reported both domestically and overseas about the impact of the G-Rh2on A549through the pathway of apoptosis induced by Bcl-2family, and the ERK pathway of the MAPK family.Our experiment used human lung adenocarcinoma A549cell as the research object. Firstly we used the MTT method to detect the cell proliferation result after using G-Rh2to treat A549under different concentration and time; and then we applied the Flow Cytometry Assay (FCM) method to detect the changes in cell cycle; finally, from the protein level, we used the Western Blotting method to test a specific protein expression level changes and explore its anti-tumor effects through the key proteins and molecular signal transduction pathway. We hope to further clarify the anti-tumor mechanism of G-Rh2through research and experiments, and promote the development and more extensive clinical application of G-Rh2anti-tumor drugs.Key results of the experiments:1, G-Rh2has obvious proliferation inhibition effect on A549, depends on concentration and time, median inhibitory concentration (IC50) is48u mol/mL2, After Using G-Rh2treated A549cells, flow cytometry revealed that in G1phase cell ratio increased, in S phase cell ratio decreased, depends on concentration and time.3, Western boltting assay for detection of Bcl-2series and ERK series of protein expression changes, with the β-actin as the internal reference control. Bcl-2expression decreased, Bax enhanced expression; Bid and Cyto-c expression were increased. P-ERK, ERK expression were decreased.Hint:1, G-Rh2can inhibiti cell proliferation of A549cell through G1cell cycle arrest,2, G-Rh2lowered the Bcl-2expression level, increased the expression of Bax, up-regulated of expression level of Bid, and increased the release of cyto-c to induce apoptosis of A549cells.3, G-Rh2may reduce the expression level of P-ERK and ERK to inhibit the proliferation of A549cells.