Detection Of Chromosomal Aberrations In Myelodyspiastic Syndromes By Using Fluorescence In Situ Hybridization

Objective: To compare the diversity of fluorescence in situ hybridization (FISH)technique and conventional cytogenetic analysis (CCA) in the detection ofmyelodysplastic syndrome (MDS). To investigate the distribution of chromosomalaberrational karyotype in MDS subgroups, and study the siginificance of karyotypeanalysis in the International prognostic Scoring system of MDS. Methods: CCA andFISH technique were used to analyzed the bone marrow cells in25cases of MDSpatients.All the patients were classified by FAB and WHO standard and scored by theInternational Prognostic Scoring System. Results:1.Using CCA,7patients (28%) withkaryotype abnormalities in25case of MDS patients were found, among them with5q-、-7、7q-、20q-、+8detection is given priority to.However,15patients (60%) withkaryotype abnormalities were found using FISH. FISH detected5q-in1additional cases,-7in1additional cases,7q-in2additional cases,20q-in3additional case,+8in3addition cases in addition to confirming abnormal karyotypes found by CCA.There wassignificant difference between the two techniques in detecting abnormal karyotypes inMDS (P＜0.05).2.FISH in the FAB classification and WHO classification each insubtypes of chromosome abnormaly detection rate higher than CCA, FISH detected in15q-that was not found by CCA,according to5q-syndrome can independent named;3.FISH detection for the standard of CCA International prognostic Scoring system resultshave influence, through the FISH detection increased obviously in the risk of patientswith low risk stratification. Conclusion: The fluorescence in situ hybridization is moresensitive for detection of chromosome abnormalities in MDS patients than that of CCA.The two methods combination can increase the positive rate of chromosome abnormality in MDS patients.FISH could improve conventional cytogenetics, and it plays animportant role in the diagnosis, prognosis in patients with MDS.