Study On Damage Mechanism Of Chronic Arsenic And Fluoride In Brain Of Rats

Objective：To investigate the biological effect and function mechanism of sodiumarsenite（NaAsO2）and sodium fluoride（NaF）in central nervous system of rats and providethe basis for further study. Method：Rats behavior that they learned to perform a waterlabyrinth task and Platform experiment；Neurotransmitters（DA，5-HT，Ach）and enzyme（SDH，LDH，MAO） levels were tested by enzyme linked immunosorbent assay；DNAdamage was observed by single cell gel electrophoresis（SCGE）. Results: There were nosignificant difference was seen in swimming duration among0mg/(Kg.d），2mg/(Kg.d）arsenite group and100mg/kg fluoride group，while rats in10mg/(Kg.d） arsenite groupand500mg/(Kg.d） fluoride group made notable more swimming time（P＜0.05）；Neurotransmitters（DA，5-HT）level significantly deceased compared with the controlgroup（P＜0.05），and significantly difference in MAO level were found in high dose groups；The10mg/(Kg.d） arsenite group and500mg/(Kg.d） fluoride showed significantdamage on DNA（P＜0.05）. Conclusion：The results demonstrate that Arsenic andfluoride exposure may develop an adverse effect on the learning capacity of rats，this maybe caused by Monoamine Neurotransmitters levels alteration and DNA damage in brain.The results also showed the interaction in the combination group was not found，it wassimply additive.