Genetic Polymorphism In TNF-βA252G And The Expression Level Of TNF-β In Plasma And Tissue And The Relationship Of Lymphoma Research

Objective:To investigate Genetic Polymorphism in TNF-βA252G and the expressionlevel of TNF-β in plasma and tissue and the relationship of lymphoma.Methods:(1)Multiple SNaPshot technology was used to assess genetic polymorphism inTNF-βA252G and the risk of subtypes of lymphoma in97cases peripheral blood samples.(2)Enzyme-linked ImmunoSorbent assay(ELISA)and Immunohistochemical techniquewere respectively used to assess the expression of TNF-β in97cases plasma samples andparaffin tissue of subtypes of lymphoma. Collecting85healthy control group peripheralblood samples and10cases of lymph nodes reactive proliferation paraffin tissuesspecimens were served as controls; Analysis the expression of TNF-β in plasma and tissueof subtypes of lymphoma and the relationship of clinical pathological features.Results:(1)TNF-βA252G gene locus A allele frequency(83.34%)is higher than the controlgroup(62.94%),but the G allele frequency(16.66%)is lower than the control group(37.06%),the differences are statistically significant(P<0.05).Therefore,TNF-βA252Ggene locus G allele may reduce the risk of HL(OR=0.34,95%CI:0.11-1.04,P=0.046).(2)TNF-βA252G gene locus genotype and allele distributions in non-Hodgkin’sLymphoma(NHL)compared with the control group, the differences were not statisticallysignificant(P>0.05).(3)TNF-βA252G gene locus genotype and allele distributions in thecases of Han nationality and minority group compared with the control group,thedifference was not statistically significant(P>0.05).(4)TNF-βA252G gene locus A/Agenotype distribution in the HL(66.67%)is higher than SLL/CLL(23.08%),but thedistribution of A/G genotype and G/G genotype(33.33%)are lower than SLL/CLL(76.92%),the difference are statistically significant(P<0.05).(5)Expression of TNF-β in plasma of HL,DLBCL,PCN,SLL/CLL,FL and PTL were higher than control,TNF-β inthese lymphoma were significant difference(P<0.05),others group had not significantdifference (P>0.05);There was no relationship between TNF-β expression of plasma andclinical features in lymphoma(P>0.05).(6)Expression of TNF-β was high inHL,DLBCL,PCN,SLL/CLL, FL and PTL,while their expression was low and weak inLBL and NK/TCL,expression of TNF-β in tissue of subtypes of lymphoma had notsignificant difference(P>0.05); Expression of TNF-β in tissue had significant differencebetween low risk group(35.71%)and middle~high risk group(70.45%)of InternationalPrognosis Index (P<0.05).(7)Genetic polymorphism in TNF-β A252G and the expressionof TNF-β in plasma and tissue of subtypes of lymphoma had no correlation.Conclusion:(1)TNF-βA252G gene locus G allele may reduce the risk of HL,G allele maybe protective factors of HL. Conversely,TNF-βA252G gene locus A allele or A/Agenotype may be associated with the HL.Also needs to be further research confirms thisconclusion.(2)Genetic polymorphism in TNF-β A252G have not associated with risk ofNHL and nation.(3)A/A genotype distribution of TNF-βA252G gene loci in subtypes oflymphoma have associated with HL,but A/G genotype and G/G genotype have associatedwith SLL/CLL.(4)Expression of TNF-β have associated with of middle risk~high riskgroup of International Prognosis Index,it have a certain reference value in the prognosisof lymphoma.(5)TNF-β had been found various degree expressions in tissue and plasma oflymphoma,TNF-β play a role in the development of lymphoma,but the expression ofTNF-β in subtypes of lymphoma may exist different mechanisms, we need to further studydetail mechanism.