Preparation, Characterization And Cytocompatibility Of Novel Three-dimensional Composite Scaffolds Via MSLDM(Multi-spraying Low-temperature Deposition Manufacturing) For Bone Tissue Engineering

Objective: To investigate the hydrophilicity, degradation rate and in vitrobiocompatibility of biomimetic scaffolds coating with geltin, so that provide evidence foranimal experiments. Methods: Two groups were examed with which gelatin-modifiedPLGA/β-TCP as the experimental group and without modification of the originalscaffolds as a control group. Hydrophilic, the degradation rate, protein adsorption rate ofscaffolds were detected. The BMSCs were seeded in two scaffolds, the cell adhesion rate,cell proliferation rate were measured. Cell adhesion in scaffolds for in vitrocytocompatibility was observated via scanning electron microscopy (SEM). Results:Experimental results showed that the hydrophilic rate of gelatin-coated scaffolds washigher than the original scaffolds at1sthour and168thhour (P<0.05), in vitro degradationrate of two scaffolds were not differ significantly (P>0.05), the protein adsorptioncapacity of coated-scaffolds was significantly higher than the original scaffolds (P<0.05),the cell adhesion rate of coated-scaffolds was significantly higher than the originalscaffolds at12thhour and24thhour (P<0.05), the cell proliferation rate ofcoated-scaffolds was significantly higher than the original scaffolds at6th,8th,10thdays(P<0.05). Conclusion: Experimental results showed that the hydrophilic andbiocompatibility of gelatin modified PLGA/β-TCP was improved, in vitro degradationrate was not affected. Our results give insight for bone tissue engineering study ofrepairing bone defects.