| Dopamine participates a lots of physiological activities in the body’s as an importantneurotransmitter in the mammalian and human. The production of many diseases of humanhas a close relationship with the change of content of dopamine. Therefore, to develop of akind of sensitive, reliable, fast detection method for the determination of the concentrationof dopamine in the body is so important for the analysis of dopamine in vivo drug anddiagnosis and treatment of the disease related to its metabolic abnormalities，and has greatsignificance in clinical. Electrochemical determination method was studied because of itsgood selectivity, high sensitivity, low detection limit, there were a lot of scholars to researchit.A high sensitivity and high selectivity chemically modified electrode was prepared bymodified the glassy carbon bare electrode with carbon nanotubes, Nafion film, SiC in thispaper. The modified electrodes were characterized by EDS, SEM and cyclic voltammetry.The results shows that the electrodes were successfuly modificated with carbon nanotubes,Nafion film, SiC, and the results were satisfactory, the experinceital were optimized. In theSolution （pH=7.5）, Dopamine Hydrochloride were determined with the differential pulseanodic stripping voltammetry （DPASV）. The results showed that the peak current wasproportional to the concentration of Dopamine Hydrochloride in the range of1×10-7mol·L-11.3×10-5mol·L-1. The linear equation ip=0.99326+0.69534C (ipunit is μA; units ofC is×10-5mol·L-1), the correlation coefficient R=0.99763, the minimum detection limit3.4×10-8mol·L-1（S/N=3）, and the scope of the recovery rate was100.4%to105.2%. And thestandard solution with1.0×10-5mol·L-1, and samples were determined, the experimentalresults showed that the chemically modified electrode possessed with good reproducibilityand accuracy. The experiment also investigated the influence of ascorbic acid and uric acidin the determination process, the results were found that the electrode on the determinationof dopamine hydrochloride was satisfactory with high stability, low detection limit,anti-interference ability, and it suited for the determination of Dopamine Hydrochloride. |
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