Study On Expression And Mechanism Of NOTCH Signaling Pathway Receptors In Invasive Bladder Cancer

Objective: To study expression and mechanism of NOTCH signaling receptors ininvasive bladder cancer and analize the cell biology alteration of T24cells with over-expression of NOTCH1.Methods: The expression of NOTCH receptors and HES1, PTEN was detected in36cases of invasive bladder cancer tissues and10cases of non-tumor bladder samples byReal Time q-PCR and Western Blot. Then NOTCH1-ORF plasmid and its blank vectorpCMV6-Entry were transfected into T24cells respectively, the expression of target genesmentioned above was measured by Real-Time q-PCR and Western Blot. In addition, cellproliferation and cell invasion was analyzed respectively by Transwell assay.Results: Compared with non-tumor bladder samples, higher levels of both mRNAand protein of NOTCH1and HES1were detected in invasive bladder cancer tissues,while PTEN expression was lower (P <0.05), and there was no statistically significantdifference about expression of NOTCH2,3receptors. Measured by Real-Time q-PCRand Western Blot in NOTCH1-overexpressed T24cells, HES1expression wassignificantly higher than the blank vector control group, while the expression of PTENwas decline (P <0.01). MTS assay showed that NOTCH1-ORF transfection obviouslypromoted cell proliferation in T24cells(P <0.01).Transwell assay showed thatNOTCH1-ORF transfection obviously promoted cell invasion in T24cells(P <0.01).Conclusion: NOTCH1gene might function as an oncogene by regulating HES1/PTEN in invasive bladder cancer, and its aberrant activation promoted cell proliferation.