The Influence Of Peroxisome Proliferator-activated Receptor Gamma And Adiponectin In Liver Fibrosis

Objective:Observing the effect of Peroxisome proliferator-activated receptor (PPAR)γ on the biological characteristic，the expression of adiponectin of rat hepatic stellatecells（HSC-T6）and its inhibition of liver fibrosis.Our resusts may provide basis forclinical use of PPARγ on anti-fibrogenic.Methods：The rat hepatic stellate cells(HSC-T6) were put into the DMEM with highglucose which contain10%FCS until the cell adherent,and synchronized by serumstarvation for24hours. The rats were divided into four groups: blank control group(cultured in DMEM with high glucose containing10%FCS), stimulated byrosiglitazone (10μmol/L), blocked by GW9662(1μmol/L), rosiglitazone+GW9662(10μmol/L rosiglitazone+1μmol/L GW9662). All groups were cultured for24hoursrespectively，①The status of HSC proliferation was measured by the MTT assay.②the level of adiponectin, type I collagen, matrix metalloproteinase2(MMP2) andmatrix metalloproteinase inhibitor2(TIMP2) in cell culture supernatant weredetected by ELISA.③RT-PCR detect the expression of adiponectin and PPARγmRNA.④The protein levels of adiponectin and PPARγ expression were evaluated bythe Western blotting method.Results：1. MTT assay results showed that rosiglitazone group of hepatic stellate cellproliferation rate is significantly reduced compared with other groups (P<0.01).2.ELISA results showed that the expression of type I collagen and MMP inrosiglitazone group were decreased compared to rosiglitazone+GW9662group,GW9662group and blank control group (P<0.01), the expression of adiponectin andTIMP2in Rosiglitazone group is significantly higher than the other three groups(P<0.01).3. RT-PCR results showed that the expression of adiponectin and PPARγmRNA in rosiglitazone group was significantly increased compared withrosiglitazone+GW9662group, GW9662group and blank control group (P<0.01),And there is significant correlation between adiponectin and the expression ofPPARγmRNA (index of correlation is0.970,p=0.000, P<0.01).4. Western blotshowed that adiponectin, PPARγ protein in rosiglitazone group was significantly higher compare with rosiglitazone+GW9662group, GW9662group and blankcontrol group (P<0.01), And there is significant correlation between adiponectin andPPARγ protein expression (index of correlation is0.966,p=0.000, P<0.01).Conclusion：rosiglitazone which is PPARγ agonist can inhibit the proliferation ofHSC, downregulating the expression of type I collagen and MMP2,upregulating theexpression of adiponectin, PPARγ and TIMP2,promot degradation of the extracellularmatrix, achieve the effect of anti-liver fibrosis.There is correlation between theinfluence of rosiglitazone on the expression of adiponectin and PPARγ.