Isolation, Purification And Identification Of Secondary Metabolites Produced By Marine Actinomyce TXC6-6and Its Strain Identification

Agricultural biological activity of a number of marine microbialmetabolites were screened for biological activity, fermentation time,metabolic production, and toxic effects of the crop factors, screeningof marine actinomycetes TXC6-6and its metabolites on plantpathogens with a relatively good inhibitionDetermine the separation and purification methods of the activecomponent included among the fermentation, after the early physicalproperties identification of active components. And then throughpretreatment of fermentation broth, solvent extrac, silica gelchromatography, thin layer chromatography，high performance liquidchromatography and other ways，four antifungal compounds wereisolated from the culture extract of strain TXC6-6by bioassay-guidedfractionation, named TXC6-6-L1, TXC6-6-L2,TXC6-6-L3-1andTXC6-6-L3-2.The spectra data of UV, MS,1H-NMR,13C-NMR,2D NMRand others will used to identify compounds TXC6-6-L1andTXC6-6-L3-2．TXC6-6-L3-2called Aurones belong to the flavonoids,it is aclass of secondary metabolites in plant, less distributed in nature, mainlypresent in the fruits of plants. biological activity compared with otherflavonoids, Aurones in the initial stage, the focus in medicine hasanti-cancer and anti-aging and other related reports. But TXC6-6-L2and TXC6-6-L3-1need further study of the separation and purificationconditions.The marine actinomycete strain TXC6-6was identified by16S rDNAgene sequence analysis and the study of morphological, physiologicaland biochemical characteristics. The results showed that the strainTXC6-6belonged to Streptomyces cinereoruber...