Anti-inflammatory, Analgesic And Anti-asthmatic Effects And Mechanism Of Alcoholic Extract Of Eleaagnus Gonyanthes Benth

ObjectiveTo study the optimum extract of total-sponins from Eleaagnusgonyanthes benth.MethodsSelect ursolic acid as control article, and Coloring by vanillin-glacialacetic acid and perchloric acid measuring absorbance bySpectrophotometer, then total sponins in Eleaagnus gonyanthes benthcalculated. The extraction rate of total-sponins was chosen as theassessment index, the alcoholic concentration, the amount of alcohol, thenumber of extraction and the extract time as examining factor and theoptimum extraction progress were selected by orthogonal design.ResultsAfter measuring the absorption peak of ursolic acid reference solutionand the test solution,541nm wavelength was selected as the determining one. Colored by vanillin-glacial acetic acid and perchloric acid, andmeasured absorbance by adopting Spectrophotometer method, and theconcentration of ursolic acid content in the range of30.4~152μg was agood linear relationship with the absorbance, the regression equation wasy=0.0052x+0.0313。 The coloration and measurement were checked:precision test: RSD=1.99%；Stability test: RSD=0.57%；Repeatability:RSD=2.48%；the average recovery of total sponins: RSD=2.78%。The result of orthogonal design show that: The optimum extractiontechnology was that using70%ethanol, the amount of which was10timesof crude drugs, to extract the crude drug2times,1.5h respectively. Theextraction rate of total-sponins was4.64（mg/g）.ConclusionThis coloration and measurement were very reliable and stable. Theoptimum extraction technology was that using70%ethanol, the amount ofwhich was10times of crude drugs, to extract the crude drug2times,1.5hrespectively. ObjectiveTo observe the acute toxicity of Study on the anti-inflammatoryeffects and mechanism of alcoholic extract of Eleaagnus gonyanthes benthand provide laboratory evidence data for the clinic usage.MethodsThe maximum administration dosage of AEEG was determined by igadministration in mice to evaluate the toxicity of AEEG.The models ofxylene-induced mice ear edema, xylene-induced dermal capillarypermeability, carrageenan-induced rat paw edema, cotton-inducedgranuloma in rat were used to study the inflammatory effectt of alcoholicextract of Eleaagnus gonyanthes benth. The contents of PGE2andhistamine in the inflammatory exudates of carrageenan-induced edema pawwas detected.Results:(1) The maximum administration dosage of AEEG was1406.277g/kg.W.(2) Compared with normal control group, AEEGtreatment group can significantly suppress the auris-swelling and dermalcapillary permeability caused by xylene in mice（P＜0.05）.There is no significant difference between AEEG-H group and dexamethasone group（P＞0.05）.(3) Compared with normal control group, AEEG treatment groupcan significantly inhibit the rat paw edema induced by carrageenan after the2h of inflammation, the inhibition can last to4h of inflammation.(4)Compared with normal control group, AEEG-H and AEEG-M group candecrease the contents of PGE2and histamine in the inflammatory exudatessignificantly.(5) Compared with normal control group, AEEG treatmentgroup can significantly suppress the granuloma hyperplasia caused bycotton in rats. the difference between AEEG-H group and dexamethasonegroup is significant(P＜0.01）. Statistical analysis was done with SPSSsystem (18).ConclusionThe AEEG is safe for oral administration; AEEG has obviousanti-inflammatory and analgesic effects, and part of its mechanism mayrelate to the inhibition of PGE2and histamine. Objective:Study on effects and mechanism of alcoholic extract of Eleaagnusgonyanthes benth on asthmatic mouse model and provide laboratoryevidence data for the clinic usageMethods:Female BABL／C mice were randomly divided into6groups(n=10ineach group)：the normal group，the model group，three mouse’s groups withlow, moderate and high AEEG respectively, and dexamethasone group.The chronic asthmatic model was established with the ovalbuminsensitization and repeated inhalation of ovalbumin aerosols. Bronchialalveolar lavage fluid (BALF) was collected to count the number of totalinflammatory and eosinophilic cells. Lung tissue sections were stainedwith hematoxylin and eosin (HE)for general morphology，with PeriodicAcid Schif (PAS)for identification of goblet cells，with masson stain fordetermination of collagen accumulation. The activity of IL-4and TGF-β1was also measured by immunohistochemistry. Statistical analysis was donewith SPSS system (18).Results:Compared with normal control group, the number of total inflammatory cells and eosinophilic cells in BALF, goblet cell hyperplasia,collagen accumulation, Wat/pbm、Wcol/pbm increased significantly. Thenumber of total inflammatory cells, eosinophilic cells in BALF, oblet cellhyperplasia, collagen accumulation, Wat/pbm、 Wcol/pbm decreasedsignificantly in AEEG-H group and AEEG-M group compared with thosein the model group(P<0.05).These difference between AEEG-H group anddexamethasone group is not significant(P>0.05). The expression of IL-4and TGF-β1were significantly inhibited in OVA induced asthmatic miceafter being treated with high and moderate dosage of AEEG(P<0.05).Thedifference of the expression between AEEG-H group and dexamethasonegroup is not significant(P>0.05).Conclusion:AEEG might suppress airway inflammation and airway remodeling bydecreasing the expression of IL-4and TGF-β1．...