Combination Of Tetramethylpyrazine And Ultrasound Microbubbles Mediate EPCS To Rat Myocardial Infarction

PART I Isolation, Culture and Identification ofBone Marrow-Derived Endothelial Progenitor Cells from RatObjective: To isolate and culture endothelial progenitor cells fromrat’s bone， also explore the conditions of their differentiation intoendothelial cells.Methods: EPCs of SD rat’ s bone were isolated bydensity-gradient centrifugation, then plated on dishes coated withfibronectin for adherent culture, and induced with VEGF、bFGF and EGF.The attached cells were detected for cell morphology,fluorescence-activated cell sorting, and the cells were also stained with Dil-ac-LDL and FITC-UEA-1.Cellular immunocytochemistry wasperformed to identify the cellsurface markers.Results: Bone marrow sources of EPCs have shape growth, strongability to proliferation. Adherent cells showed that EPCs express CD133,CD34, Flk, Ⅷ factor (vWF) in different times. The attached cells can stainDil-acLDL and FITC-UEA-1.And we also find w-k body in cytoplasm atthe late stage.Conclusions: Bone can separate marrow and cultivate EPCs by useof a density gradient centrifugation on percoll. EPCs could be differentiatedinto vascular ECs in vitro after induction culture with bFGF, EGF, VEGF,which as the identical feature as the endothelial cells (ECs). PART II Combination of Tetramethylpyrazine andUltrasound microbubbles mediate EPCs to rat myocardialinfarctionObjective: To investigate the feasibility of combination of TAM andUS of intravenously implantated EPCs.Methods: Isolation, culture and identification of EPCs from rat bonemarrow.Rats are divided into5groups after the method of myocardialinfarction prepared.(1)TMP+US+EPCs(2)TMP+EPCs(3)EPCs(4)Blank control group.（5）AMI.All the rats were sacarificed48h after EPCsimplantation.Results: Compared with other groups, TMP+US+EPCs groupshowed improving cardiac function(P<0.05), increased VEGF levels(P<0.05) and highest homing number of EPCs (P<0.05).Conclusion: Tetramethylp yrazine as well asultras o und mic rob ubb les c a n imp ro ve EPCs infarc tio n, whic hp ro vid e no vel s trategy fo r EPCs imp lantatio no f isc hemic heard is eas es.