LRP1B Expression In Pancreatic Cancer Cells And Its Significance

Objective：Through test the expression of LRP1B in normal pancreatic cell and three pancreaticcancer cells, discuss LRP1B relationship with pancreatic cancer.Methods：To pancreatic cancer cell lines（ASPC-1, PANC1, BxPC3, HPDE6C7) as the researchobject, Real Time-PCR is use to assay the expression of LRP1B mRNA.Western blot is useto assay the expression of LRP1B protein. Divided into two groups:1, normal pancreaticcells and three pancreatic cancer cell expression differences;2, three pancreatic cancer cellexpression differences between comparison.Results：Real Time-QPCR result revealed the LRP1B mRNA was expressed in four cell lines,and measured2^-a delta Ct value, x±s: PANC-11.278±0.165、ASPC-13.244±0.485、BXPC-38.448±1.140、HPDE6C717.275±0.387; the average compared with the t test andsingle factor variance analysis; P <0.05for the difference was statistically significant.Western-blot method for the detection of LRP1B protein in three pancreatic cancer celllines expressing, according to the PANC-1ASPC-1and BXPC-3’film gray analysis,LRP1B protein in the gray value and the corresponding cells in theβ-actin gray value ratioof column data table, use x±S said：PANC-10.524±0.024、ASPC-10.571±0.016、BXPC-30.721±0.019、HPDE6C71.233±0.023; the mean’s compare by the t test andsingle factor variance analysis; P <0.05for the difference was statistically significant. Theprotein and mRNA of LRP1B expressed in three pancreatic cancer cells by Western-blotmethod and Real Time-QPCR method: HPDE6C7> BXPC-3> ASPC-1> PANC-1.Conclusions：1．LRP1B is a high state of expression in normal pancreatic cell, in three pancreatic cancer cells showed low expression;2．LRP1B has tumor specificity expressed in pancreatic cancer cells.