Bile Pathogen Cultivation And Antibiotic Drug Sensitivity Analysis In Patients With Common Bile Duct Stones

ObjectiveTo analysis the distribution of bile pathogenic bacteria and the change of drug sensitivity in patients with CBDS, and to guide rational application of antibiotics.MethodsSelecting72cases of patients with CBDS from March2011to February2012,collecting bile specimens through ERCP treatment.Then pathogenic bacteria cultivation and drug sensitivity test were carried out.ResultsThere were53positive cases in72bile specimens, with a positive rate of73.61%;7cases were mixed infection, accounting for13.21%of infected patients.60strains were trained, including gram-negative bacterium45strains (75%), gram-positive12strains (20%), fungi3strains (5%); The most five predominant pathogenic bacteria were Escherichia coli (30%), Entercoccus faecium (11.67%), Klebsiella pneumoniae (10%), Enterobacter cloacae (6.67%), Pseudomonas aeruginosa (5%), Aeromonas hydrophilia (5%).The results of drug sensitivity showed the sensitive rate of gram-negative strains to imipenem, amikacin was the highest (95.45%), followed by ertapenem (94.12%), meropenem (93.18%), Piperacillin/taz (79.55%). The resistant rate of gram-negative strains to ampicillin was as high as88.57%, and to some Ⅰ～Ⅲ generation of cephalosporin>60%, to quinolones>50%respectively.The sensitive rate of gram-positive strains to vancomycin, teicoplanin linezolid was the highest(100%), followed by nitrofurantoin (80%).The resistant rate of gram-positive strains to Penicillin G was81.82%, and to ciprofloxacin70%, and to gentamycin, tetracycline, ampicillin>50%respectively.ConclusionsGram-negative strains remained the commonest pathogens in patients with common bile duct stones in this hospital. The common antibiotics sensitive rate was lower, so the monitoring of antimicrobial drug sensitivity should be strengthened in clinical. And we should choose the appropriate antibiotics according to antimicrobial susceptibility test results.