Multi-gene Methylation Testing Increased The Positive Ethylation Rate In Colorectal Cancer

ObjectiveTo study the value of detection the methylation of Vimentin、sFRP1and HPP1gene in colorectal cancer.Methods90patients with colorectal cancer,60patients with adenomatous polyp and20normal people, were included in the study. DNA was extracted from tissue samples, andthe methylation status of Vimentin, sFRP1and HPP1gene were detected byMethylation-specific PCR (MSP). The relation between clinicopathologic features ofcolorectal cancer and gene methylation were analyzed.ResultsThe methylation frequencies of Vimentin, sFRP1and HPP1were66.7%、68.9%、72.2%in colorectal cancer,53.3%、55.0%、50.0%in colorectal adenomas, and0、0、5.0%in healthy controls, respectively. The methylation of each of the three genesin colorectal cancer were higher than colorectal adenomas and healthy controls (P<0.05).The diagnostic sensitivity by combining three methylation marks was93.3%incolorectal cancer,76.7%in colorectal adenomas, which was higher than single gene(P<0.05). No significant associations existed between the methylation status of the threegenes and clinical characteristics including sex,age,tumor location, lymph nodemetastases and TNM stage (P>0.05).Conclusion:DNA methylation levels of Vimentin, sFRP1and HPP1are significantly higherin colorectal cancer, their joint detection significantly improved the positive rate of methylation. It may be one of the early diagnosis proposals of colorectal cancer.