Protection Of Selenium On Dysfunction Induced By Depleted Uranium Instillation Through Trachea In Rats

The main component of depleted uranium (Depleted Uranium) is U3O8, has thesame chemical properties with the natural uranium. Depleted uranium in addition todamage as a kind of heavy metal, but also has strong radiation injury. It has militaryuses, used to produce depleted uranium weapons bombs, depleted uranium bombsexplosion and produce large amounts of aerosols, to enter the body through breathingand cause great damage to the human body. At present, the study on depleted uraniuminjury is more, while, the research at the quantitative protection aspects is relativelyscarce.In this study, different doses of protective agents-selenium gavaged in rats, andthe established damage model of the depleted uranium intratracheal instillation, toobserve the changes of body weight, lung uranium content, peripheral blood cellparameters, organ coefficient, the parameters of lymphocyte subsets, immunoglobulin,endocrine index, blood biochemical parameters, oxidation indexs and pathologicalindicators at different time points,to evaluated of selenium on the protective effects ofdepleted uranium tracheal perfusion injury in rats, the protective measures for thedevelopment of the hazards of human depleted uranium reference system.Wistar rats were randomly divided into normal control group、exposed groups ofDU、DU+low selenium group、 DU+selenium group、 DU+high-selenium group(N=45), leaving10rats killed as0day control after exposure. Depleted uranium wasinstilled through trachea at500microgram DU per rat. Selenium were administereddaily at50μg·kg-1、200μg·kg-1、500μg·kg-1body weight from3days before DUexposure to the day were killed.Rats were killed on the7th day、14th day、28th day,respectively, and detected body weight, lung uranium content, the parameters of lymphocyte subsets, immunoglobulin, endocrine index, blood biochemical parameters,the oxidation indexs, and observed and analysised of the pathological change.At the7th day after DU exposure, rats treated by DU, body weight gaindecreased, Pulmonary uranium content decreased, white blood cells elevated, redblood cell number was significantly lower, hemoglobin and platelet levels increased,organ coefficients changed, T lymphocyte and its subpopulation decreased significantly.serum immunoglobulin IGg significantly reduced, Thyroid axis indicators TSH、 T3、FT3、T4and FT4decreased, Adrenal axis indicators ACTH、 ALD、COR wereelevated, Liver function parameters AST、ALT and ALB increased, Renal functionUA increased, Myocardial enzymes as CK and AST was significantly increased,bloodlipids were lower,TG、HDLwere significantly higher, oxidation indicator MDAincreased, GSH-px was significantly lower, Fibroblast proliferation, Interval widenedthe alveolar septa, peribronchial infiltration of lymphocytes,alveolar spaces orBronchioles intraluminal fibrin exudation, Alveolar septum fracture, red blood oozing,collagen deposition,8-hydroxygwunosine and Caspase3expression significantlyincreased, the severity of selenium protection group significantly reduced, collagendeposition and the expression of8-hydroxygwunosine and Caspase3significantlyreduced.DU exposure after14days, red blood cells,lung index、CD3+、CD3+CD8+, T3、T4、FT4、AST、ALT、TP、Cre、HDL、GSH-px、the severity of collagen deposition,the expression amount of8-hydroxygwunosine and Caspase3compared to the normalcontrol group were obviously differences. Low doses of selenium protective groupsparameters improved significantly.DU exposure after28days, white blood cells、the liver indexs、CD3+CD4+、CD3+CD8+、FT4、Cre、MDA、GSH-px、collagendeposition、the expressionamount of8-hydroxygwunosine and Caspase3compared to the normal control group were obvious differences. DU+selenium group parameters were near to normallevels, which improved significantly.Conclusion, DU intratracheal instillation in rats with systemic, functional andorganic damage, and the damage to the lung was the most serious. DU caused weightloss, diperipheral blood parameters disorder, Immune function suppressed, endocrinedisorder, liver dysfunction, renal dysfunction, oxidative damage. and the pathologicaldamage of the lungs was the most serious. Selenium protection group compared toDU controls improved significantly. Therefore, Selenium has protective effect ondysfunction induce by depleted uranium.