Expression Of Ki-67and Livin Proteins In Human Non-Small Cell Lung Cancer

Background: Lung cancer is the most common primary malignant tumor of thelung, particularly in industrial countries around the world, lung cancer incidenceand mortality are rising rapidly, its occurrence is related to cell proliferation andapoptosis imbalance. Ki-67is a DNA-binding nuclear protein, expressed in proliferatingcells of the cell cycle, but not expressed in quiescent, which is highly related to tumorcell proliferation and prognosis of cancer patients. Livin is discovered to be a novelmember of the inhibitor of apoptosis protein family, which is absent in most terminalorganization (except placenta), but highly expressed in most cancer cells. Livin caninhibite apoptosis and promote cell proliferation. When the balance is broken,tumorhappened. Livin and Ki-67may be potential targets for novel diagnosis andtherapies in NSCLC.Objective：In this study，immunohistochemistry was used to investigate expressionsituation of Ki-67and Livin proteins in NSCLC specimen and pericarcinomatoustissues, ananlyze its ralationship with clinical pathological factors,and investigate theassociativity between the expression level of Ki-67and Livin in NSCIC tumortissue．The purpose was to study their roles in invasion and metastasis of NSCLC andprovide new theoretical foundation for forecasting the early diagnosis，prevention andclinical treatment, judging prognosis of NSCLC． Methods： Samples of54NSCLC and13pericarcinomatous tissues were detected bystreptavidin peroxidase immunohistochemistry of Ki-67and Livin proteinsexpression.SPSS (18.0)statistical software was applied for data analysis.A P-value lessthan0.05were condidered signficnt.The correlation among the expression of Ki-67andLivin and the major clinical pathological characteristics of NSCLC were analyzed byChi-square test.Results:1.The total positive expression rate of Ki-67protein was72.2%in NSCLC，significantly higher than in pericarcinomatous tissues (15.4%), the difference wasstatistically significant (P <0.05)。 High expression was associated with Grade (poorlydifferentiated tumors:89.7%vs.52.0%)(P <0.05). Positive expression rate of Ki-67in Ⅰ+Ⅱ stages and Ⅲ stages was59.3％and85.2%respectively, the difference hadstatistical significance(P <0.05). Positive expression rate of Ki-67was50.0%in thenon-lymph node metastasis group and83.4%in the lymph node metastasis group，thedifference had statistical significance(P <0.05). There was no relationship betweenKi-67and histology, age and sex(P>0.05).2.The total positive rate of Livin protein was66.7%in NSCLC，significantly higherthan in pericarcinomatous tissues (23.1%), the difference was statistically significant (P<0.05). Positive expression rate of Livin was38.9%in the non-lymph node metastasisgroup and80.6%in the lymph node metastasis group，the difference had statisticalsignificance(P <0.05). There was no relationship between Livin and pathologic TNMstage, grade, age,histology and sex(P>0.05).3.There was a significantly positive correlationship between Ki-67expression andLivin expression in NSCLC(r=0.439, P <0.01).Conclusion：1.Ki-67was mainly localized in the nucleus, is highly expressed inNSCLC. There was no relationship between Ki-67and grade, pathologic TNM stage, lymph node metastasis.There was a relationship between Ki-67and lung cancerdevelopment and metastasis, Ki-67can provide new theoretical foundation forforecasting the early diagnosis，judging prognosis of NSCLC and be a means of genetherapy.2.Livin protein is highly expressed in NSCLC,but low expressed or absent inpericarcinomatous tissues. Its high expression was associated with adenocarcinoma,which is expected to be a molecular marker for lung cancer treatment.3. There was a significantly positive correlationship between Ki-67expression andLivin expression in NSCLC,and some synergy in the development of NSCLC. Thecombined detection of Ki-67and Livin can guide early clinical diagnosis, predictthe trend of lymph node metastasis, determine and improve the prognosis and treatmentplan.