Expression And Significance Of P-EGFR、P-ERK And NF-κB Proteins In Middle Ear Cholesteatoma Epithelium

Objective:To determine the expressions of P-EGFR (phospho-epidermal growth factor receptor), P-ERK (phospho-extracellular signal-regulated kinase) and NF-κB (nuclear factor kappa B) in middle ear cholesteatoma and their relationship, and explore their roles in the pathogenisis of cholesteatoma.Methods:SP immunohistochemical assay was used to detect expressions of P-EGFR, P-ERK and NF-κB proteins in30human middle ear cholesteatoma tissues and15normal skin specimens collected from the external ear canal.Results:The positive expression rate of P-EGFR protein in middle ear cholesteatoma epithelium (63%) is higher than that in normal skin specimens (20%) collected from the external ear canal(P<0.01). The positive rates expression of P-ERK protein in middle ear cholesteatoma epithelium and normal external ear canal skin were72%and20%respectively, and the difference between the two groups was statistically significant (P<0.01). The positive expression rates of NF-κB protein in middle ear cholesteatoma epithelium and normal external ear canal skin were60%and15%respectively, and there was also a statistical significance between the two groups (P<0.01). In all the30cases of human middle ear cholesteatoma epithelium, there are obvious positive correlation between P-EGFR, P-ERK and NF-κB (P<0.01).Conclusion:The abnormal expressions of P-EGFR, P-EPK and NF-κB proteins may have a close relationship with highly proliferation of epithelial tissue in cholesteatoma. Simultaneously there are obvious positive correlation between P-EGFR, P-ERK and NF-κB, which indicating that ERK signaling transduction pathway serves an critical function in the proliferation of cholesteatoma.