Significance Of The FISH Technology In HTERC Gene And C-myc Gene Detection For The Screening Of Cervical Cancer

ObjectiveTo investigate the correlation between hTERC gene and c-myc gene and the development of cervical carcinoma and the significance of the detection of the two genes in the screening of cervical cancer.MethodsOne thousand cytological specimens were randomly collected in the Gynecology and Obstetrics clinic of the Second Xiangya Hospital of Central South University from Nov.2010to Feb.2011.All the specimens had undertaken Thinprep Cytology Test (TCT), hTERC (FISH technique) and C-MYC (FISH technique) tests. Patients who had any positive result of the three tests further underwent the colposcopy and cervical biopsy, where the histopathology was regarded as the gold standard. According to the final pathological results,213cases out of the1000have been enrolled the TCT, hTERC gene and C-MYC gene detection, and histopathological examination in the study.Results1.Positive rates of hTERC were5.8%,20.0%,50.0%,81.0%and100.0%respectively in normal/inflammation, cervical intraepithelial neoplasia grade1(CIN1), grade2(CIN2), grade3(CIN3) and squamous cervical cancer (SCC) group. Positive rates of hTERC were8.6%,21.3%,51.9%,72.0%and100.0%respectively in NILM, ASCUS, LSIL, HSIL and SCC based on the cytological diagnoses. The elevatory amplification rates of hTERC gene were consistent with the severity of lesions diagnosed by cytological test and histopathological examination (P<0.001). Positive rates of hTERC gene were positive correlation with severity of lesions diagnosed by cervical cytological test or histopathological examination (P<0.001).2.Positive rates of C-MYC were9.20%,13.30%,45.80%,76.20%and88.90%respectively in normal/inflammation, cervical intraepithelial neoplasia grade1(CIN1), grade2(CIN2), grade3(CIN3) and squamous cervical cancer (SCC) group. Positive rates of C-MYC were13.60%,18.70%,40.70%,72.00%and80.00%. respectively in NILM, ASCUS, LSIL, HSIL and SCC based on the cytological diagnoses. The elevatory amplification rates of C-MYC gene were consistent with the severity of lesions diagnosed by cytological test and histopathological examination (P<0.001). Positive rates of C-MYC gene were positive correlation with severity of lesions diagnosed by cervical cytological test or histopathological examination (P<0.001).3. Both hTERC gene test and C-MYC gene test showed approving sensitivity, specificity and negative prediction rate on the condition of high grade of cervical lesions. The sensitivity was obviously superior to TCT, among which hTERC gene test showed the highest sensitivity (74.6%)。TCT together with hTERC or C-MYC gene test had higher sensitivity, negative prediction rate and Youden’s index, whereas lower rate of missed diagnosis compared to single test alone. The area of ROC of TCT together with hTERC or C-MYC gene test, and TCT along was0.851,0.821,0.704respectively. The area of joint tests was larger than TCT alone (p<0.05).Conclusions1. The amplification rates of hTERC gene and C-MYC gene were positive associated with the severity of lesions diagnosed by cervical cytological test or histopathological examination. They can be served as significant biogenetic markers in predicting the development of cervical lesions and distinguishing low-grade lesion from high-grade.2. TCT together with hTERC or C-MYC gene test by FISH technology can be used in the diagnosis of the screening of early cervical cancer.