The Study Of Relationship Between Endothelial Lipase Gene Polymorphism And Atherosclerotic Cerebral Infarction

Objective To explore the association between EL gene polymorphism and atherosclerotic cerebral infarction(ACI),and the possible influence of these SNPs on blood lipid level and(arteriosclerosis, AS)Methods Polymerase Chain Reaction (PCR), Restriction Fragment Length Polymorphism (RELP) and DNA sequence were performed to identify the distributions of allele and genotype frequencies of SNP polymorphism in EL gene in266patients with ACI and91healthy controls. Serum total cholesterol (TC), triglyceride concentrations (TG), Low-density lip-oprotein cholesterol (LDL-C) and High-dens-ity lipoprotein cholesterol(HDL-C) were determined by an enzymatic method with autobiochemistry instrument.and carotid atherosclerotic plaques was examined by neck vascular ultrasound.Results:1. Single-Point genotypes and alleles distributions in Thr111Ile and Gly26Ser of the EL gene were not statistically different between the ACI and control groups(P>0.05).2. The carriers of the TT homozygote in the ACI and control groups had a higher level of HDL-C compared with CC homozygote (P<0.05). there were not statistically different level of HDL-C between the ACI and control groups (P>0.05). 3. The carriers of the TT homozygote exhibited significant higher level of HDL-C as compared with CC homozygote carriers in males ACI and female control groups of Thr111Ile(P>0.05). There were not statistically different level of HDL-C between the GA genotype and GG of Gly26Ser in female ACI and male control groups(P<0.05).4. The presence of the TT genotype of Thr111Ile was associated with0.343greater odds of AS compared with CC genotype(95%CI:0.129,0.913; P=0.032).Conclusion1. The Thr111Ile polymorphism of EL gene may be associated with the level of HDL-C.Carriers of the TT homozygote may be involved in high level of HDL-C.2. There is no relationship between the Thr111Ile and Gly26Ser polymorphism of EL gene and ACI in Han population in Hunan Province.3. The Thr111Ile polymorphism of EL gene may be associated with the AS, and the carriers of the TT homozygote may be a protective factors of AS.