The Biocompatibility Studay Of Xenogeneic Bone Scaffold Materials Prepared By Different Ways

Objective: To detect the biocompatibility of xenogenic bone scaffold materialsprepared by three different ways. Methods: Acute toxicity test, pyrogen test,intramuscular stimulation test, subcutaneous implantation test and cytotoxicity test wereused to evaluate the biocompatibility to sheep vertebral bone xenograft materials whichwere prepared through physical, chemical and physical-chemical combined methods.Results: Acute toxicity test: No toxicity symptom was shown for either physical orphysical-chemical combined groups or control group within7days; The mild dyspneasymptoms was shown for chemical group animal early, but no death. It shows there wasno toxicity in materials prepared through physical, physical-chemical combined methods;The chemical groups showed slight toxicity. Pyrogen test: The temperature changesbefore and after the experiment showed statistically significant difference (P＜0.05) forphysical, chemical and physical-chemical combined groups. It shows there was nopyrogen reaction in materials prepared through physical, physical-chemical combinedmethods; While there was pyrogen reaction in materials prepared through chemicalmethods. Intramuscular stimulation test: There was no clear erythema, edema andnecrosis at the injection site of the skin. It means there was no irritation in physical orphysical-chemical combined groups; The chemical group showed slight erythema at48hand72h at the injection site of the skin, The chemical group was slightly irritating.Subcutaneous implantation test: Histological observation discovered a small amount oforderly osteoblasts in physical-chemical combined group materials around, meanwhile asmall amount of bone, it suggests that there was a tendency for ectopic bone formation.However, there was no such sign of orderly osteoblasts in physical and chemical groups.Cytotoxicity test: MTT method proved that material immersion of physical, chemical,physical-chemical combined and control groups with culturing of BMSCs in12days hasstatistically significant difference (P＜0.05) among the other groups except for physicalgroup and physical-chemical combined group and control group (P＞0.05). The cells on the physical group and physical-chemical combined group materials could well adhereand proliferate and grow; The cells on the chemical group material was restrained,scaffold pores without cell adhesion. Conclusion: The xenogeneic bone materialsprepared in the study presented through physical-chemical combined methods have bettercytocompatibility. However, scaffold materials prepared through physical or chemicalmethods have poor biocompatibility, thus they are not qualified for the safety standardsof biological materials.