| Objective:Diabetes mellitus (DM) is one of the common chronic diseases. Its chronic complications can involve in the whole body tissues, reduce the survival of the patients, the life quality and increase the economic burden. Traditional therapy is to control blood sugar and treat the complications, but it couldn’t prevent the chronic complications progress. In recent years, our previous study found that the immunity and inflammatory mechanisms play an important role in DM and the occurence of chronic complications. Cordyceps sinensis is an artificial caterpillar fungus, in clinic, is used as an immune adjustment agent, in this experiment, the morphological changes of the skeletal muscle tissue were observed to evaluate the effect of Cordyceps sinensis in streptozotocin (STZ) induced diabetic rats, the inflammation factors including TNF-α, MCP-1,IL-6expression and immunoglobulin deposition, exploring the role of the immune and inflammatory lesions in the corresponding organs for DM pathogenesis, to provide theory basis for DM prevention and treatment.Materials and methods:Ninety males SD rats,10rats was randomly picked up as normal control group, and the rest were assigned to experimental group, wich was STZ induced diabetic rats, the experimental group were randomly divided in to small doses of cordyceps sinensis group(0.6g/kg/d), middle dose of cordyceps sinensis group(2.5g/kg/d), large doses of cordyceps sinensis group (5.0g/kg/d), pioglitazone group (4mg/kg/d) and diabetes group, after10weeks treatment,all of the rats were sacrificed, the blood and urine specimen were collected, the renal function, blood fat and microalbuminuria were detected respectively. Through HE, MAS SON, PTAH dyeing observing the pathological changes with light microscopy, IgG deposition was detected by immune fluorescence, the TNF-α, MCP-1,IL-6expression were observed through immunohistochemistry.Results:After the dose of45mg/kg STZ intravenous in the rats, when the blood sugar was increased morethan16.7mmol/L, indicated that the model of diabetic rats was successed. The blood sugar level in the diabetic rats were significantly higher than that in the normal control group (P<0.05), and no significant differences among diabetic groups (P>0.05). Compared with DM group, the biochemical indicators were decreased in PIO and cordyceps sinensis group (P<0.05), but there were no differences among treatment groups (P>0.05). The inflammatory cell infiltration, skeletal muscle atrophy, obvious fibrosis and IgG sedimentation were found in DM group, while the pathological changes were lighter and the TNF-α, MCP-1,IL-6expression were also decreased in the treatment groups.Conclusions:STZ induced DM rats may developed multiple organ damage associated with immunity, immunoglobulin abnormal deposition in skeletal muscle suggested that the immunity may play a key role in the DM chronic complications and progression. Cordyceps sinensis and PIO have some immuno-regulation or immuno-suppression effects, may reduce the skeletal muscle lesions and the skeletal muscle immunoglobulin precipitation, suggesting that immunosuppression treatment may prevent or delay the damage of the diabetic skeletal muscles. TNF-α and MCP-1,IL-6protein expression increased may promote the diabetic skeletal muscle lesions in STZ-induced diabetic rats. Cordyceps sinensis and PIO treatment can inhibit the TNF-α, MCP-1,L-6protein expression of the skeletal muscles in the DM rats, the possible mechanism seems to reduce the Immune Complexes deposition and suppress the inflammation cell factors such as TNF-α, MCP-1,IL-6expression in different organs and tissures, thus inhibit the immune inflammation. |
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