The Effects Of Didang Decoction On The Expression Of ICAM-1、VCAM-1in Diabetic Rats

Objective:Through inducing the formation of STZ diabetic rat model, we explored the influence of Didang decoction on rats with diabetic by observe ICAM-1and VCAM-1expression in lower extremity vascular and renal of rats.Methods:After being injected streptozotocin (STZ) into the caudal vein and fed with the high fat diet, diabetes model rats were divided into normal control group, model control group, Didang decoction late group, Didang decoction interim group, Didang decoction early group, atorvastatin calcium group and the rosiglitazone group. We observed the growth status, body weight, blood glucose and blood insulin, morphological changes of, the immunohistochemical and pathological changes of ICAM-1and VCAM-1, and explored the possible mechanism of Didang decoction’s influence on diabetic.Results:STZ-induced diabetic rat model was established successfully. In end of the experiment (24weeks from the start point), Compared with the normal control group, the blood glucose of the rats of STZ induced diabetes model was significantly increased; compared with the model control group, the blood glucose of the rats in rosiglitazone group was reduced, and the blood glucose of the rats in Didang decoction intervention groups and simvastatin group did not change significantly; compared with the normal control group, the femoral artery and kidney tissue stained by HE of the rats in model control group had irregular structure, disordered cells, and some cells prominent from the inner limiting membrane observed under light microscope, indicating that these tissue of diabetic rats pathologically changed,compared with the model control group, Didang decoction interim group, Didang decoction early group, Simvastatin group and rosiglitazone group pathologically changed lighter. And the ICAM-1and VCAM-1expressed in the femoral artery and kidney tissue of the rats in model control group after stained through immunohistochemical method increased significently compared with the normal control group, meanwhile the Didang decoction interim group, Didang decoction early group, Simvastatin group and rosiglitazone group showed a lighter expression. Conclusion:In this study, we observed the vascular disease of lower extremities in diabetic rats based on STZ-induced diabetic rat model, and found that the early and interim intervention with Didang decoction in the rats could affect the occurrence and development of chronic complications of diabetes. After the early and interim intervention with Didang decoction, the vascular of lower extremities and kidney of the diabetic rats are significant protected and the procedure of the pathogenesis of diabetic slowed down; and progress of diabetic complications is relatively minor compared with the model control group; this may be associated with the role of Didang decoction through reducing the expression of ICAM-1and VCAM-1in diabetic rats, and so on.