| Objective In order to evaluate the role of Dickkopf-1(DKK-1)level in the pathogenesis of rheumatoid arthritis (RA) and the joint erosivity, to provide new ideas for early detection and eariy treatment of RA bone destruction and to judge the degree of bone erosion, the serum level of DKK-1and other factors in RA patients were investigated, and the relationship between DKK-1and other clinical and laboratory findings were analyzed simultaneously.Methods The serum of65RA patients and20healthy controls were collected. Based on the activity index of RA, the65RA patients were divided to35active RA group and30inactive RA group, and on the duration of disease were divided into early RA group and late RA group, and on the ACR diagnostic criteria and joint damage in patients with X-ray stage, they were divided to erosive RA group and nonerosive RA group. The concentration of serum DKK-1in the RA Patients and healthy controls was measured by sandwich enzyme-linked immunosorbent assay(ELISA). Simultaneously, the clinical feature and laboratory index like blood cell count, rheumatoid factor(RF), anti-cyclic citrullinated peptide(CCP) antibody, erythrocyte sedimentation rate(ESR), C-reaetive protein(CRP)and immunoglobulin(IgG, IgA, IgM), the duration of disease, morning stiffness.the number of painful joints, the number of swollen joints, X-ray stage of joint damage and disease activity score28were recorded. SPSS17.0statistical software was used to analyze differences between the data of each group.Results1. The levels of serum DKK-1was significantly higher in RA patients than that in the healthy controls(15.99±11.54ug/L vs7.66±2.89ug/L, t=5.31. P<0.001).2. The levels of serum DKK-1in early RA group were higher than the healthy controls, but without significant difference(10.68±6.09ug/L vs7.66±2.89ug/L, P>0.05). The levels of serum DKK-1in late RA group were significantly higher than that in early RA group(18.67±13.30ug/L vs10.68±6.09ug/L. t=3.08.P<0.01).3. The levels of serum DKK-1were higher in the erosive RA Patients than that in those non-erosion RA patients(19.92±12.75ug/L vs9.69±4.89ug/L. t=4.57, P<0.001). There was no difference in the levels of serum DKK-1between non-erosion RA patients and the healthy controls (9.69±4.89ug/L vs7.66±2.89ug/L, P>0.05).4. The levels of serum DKK-1was higher in active RA patients than that in the inactive RA patients (18.69±13.33ug/L vs12.41±1.41ug/L, t=2.41, P<0.05).5. The levels of DKK-1in RA patients were positively correlated with duration of disease, morning stiffness, the number of the painful joints, the number of swollen joints and DAS28(P<0.05), and were not correlated with age, gender, Hb, PLT, ESR, IgG, IgA, IgM, CRP, anti-cyclic citrullinated peptide (CCP) antibody and RF and so on(P>0.05).Conclusion1. The levels of serum DKK-1in RA patients elevated compared to the healthy controls, which suggested DKK-1may take part in the pathology of RA.2. The levels of serum DKK-1were higher in active RA patients than that in the inactive RA patients, and showed positive correlation with the active index such as morning stiffness, the number of the painful joints, the number of swollen joints and DAS28, which suggested that DKK-1associated with disease activity.3. The levels of serum DKK-1were higher in the erosive RA patients than that in those non-erosion RA patients, and the levels of serum DKK-1in late RA group were significantly higher than that in early RA group, and showed positive correlation with duration of disease, which suggested that DKK-1was closely related to bone erosion.Its levels may be involved in the pathogenesis of RA bone destruction and probably be an indicator of predicting future arthrosis destruction. |
PDF Full Text Request