Characteristic And Cytotoxicity Of AIE Molecule-doped Silica Nanoparticles For Live Cell Imaging

ObjectiveTo study the characteristic, live cell imaging and cytotoxicity of fluorescent silicananoparticles hybridized with aggregation-induced emission luminogents(SD-FSNP).MethodsThe shape and diameter of SD-FSNP were observerd by transmission electronmicroscope(TEM) and the spectroscopy were examied by the spectrofluorometer.Hela cervical cells and NIH3T3mouse fibroblast cells were treated with variousconcentrations of SD-FSNP. These nanoparticles were utilized to stain live cells andthe cytotoxicity of the nanomaterial was investigated on cell morphology, cellviability, cell survival, cell apoptosis and cellular ROS.ResultsThe SD-FSNP were spherical, monodisperse, smooth surface and uniform size,with an average diameter of approximately100nm, and emitted strong fluorescencewith the peak of485nm. The nanoparticles selectively stain the cytoplasma regionand have no significant toxic effects on cell viability, cell survival, cell apoptosis andcellular ROS at the maximum working concentrations.ConclusionThis study indicates that the SD-FSNP is a promising biocompatible fluorescentprobe with low toxicity as a cytoplasmic stain of living cells.