To Study The Interference Effect Of Compound Sophorae Flavescentis Decoction On DOR-β-arrestin1-Bcl-2Signal Transduction Pathway In Rats With Ulcerative Colitis

Objective:To explore whether DOR-β-arrestin1-Bcl-2signal transduction pathwaymediates the intervention effect of ulcerative colitis in rats.Methods: eighty-four Sprague-Dawley rats were equally and randomly divided into sixgroups: normal control group, model group, mesalazine group, Compound SophoraeFlavescentis decoction high-dose group, medium dose group and low-dose group,(n=14).In addition to the normal control group, Ulcerative Colitis were induced withtrinitrobenzene sulfonic acid (TNBS) in rats in each group. After modeling, bloodystool,mental state and diarrhea were observed and record in the experimental rats. Two ratswere randomly selected and put to death in model groups on day3for observing colontissue pathologic changes with microscope.The rats in mesalazine group were given agavage with3mL mesalazine solution for15days,while those in the normal control groupand model group were to put the fluid into stomach with distilled water of the same volumewith the drug for15days. According to the difference of crude drug concentration ofCompound Sophorae Flavescentis decoction (0.67g/L,0.34g/L,0.17g/L), the rats inCompound Sophorae Flavescentis decoction were lavaged with3mL Chinese drugs for15days. In the16day,the remaining rats,after24hours of fasting,were executed to detect themRNA and protein expression changes of DOR, beta arrestin1and Bcl-2in the colon tissueby Real Time-PCR and immunohistochemistry respectively. And,taking colonic tissues were embedded in paraffin HE staining of colon tissue histopathological changes.Results: In the experimental rat colon tissue, the expression of, DOR, β-arrestin1and Bcl-2protein and mRNA expression were significantly different (P <0.05) and they hadstatistically significant. Compare with the normal control group,the expression ofDOR,beta-arrestin1and Bcl-2protein and mRNA was significantly increased(P<0.05) incolon mucosa in the model group. After drug treatment, compared with the mode group, theexpression of DOR, beta-arrestin1and Bcl-2protein and mRNA were significantlydecreased in colon mucosa in mesalazine group, and Compound Sophorae Flavescentis sidehigh-dose group, medium dose group and low-dose group. But the expression of DOR,beta-arrestin1and Bcl-2protein and mRNA was no significant difference betweenmesalazine group and Compound Sophorae Flavescentis side high-dose group, mediumdose group and low-dose group (P>0.05).Conclusion: the expression of DOR, beta-arrestin1and Bcl-2is elevated in experimentalulcerative colitis colon tissue. DOR-beta arrestin1-Bcl-2signal transduction pathways maybe involved in the pathological process of ulcerative colitis, and Compound SophoraeFlavescentis decoction may have a significant effect on the treatment of ulcerative colitis byregulating the signaling pathway.