In Vitro Characteristics Analysis Of Ventral Meseneephalie Precursor Cells From Sprague-dawley Rats At Embryonic Age Of11Days

Objective: To investigate the self-renewal, proliferation and differentiation of ventralmeseneephalie precursor cells(VMP),isolated from sprague dawley rats at embryonicage of11days (E11)in vitro. Methods: ventral mesencephalon (vm) cells from fetalrats at embryonic age of12days(E11) was gained by cultivated in vitro, then culturedin P1medium at the first day, P2medium from2nd to7th day, D medium from8th to14th day. Cell observation and cell count were carried out everyday. Immuno-fluorescent staining was used to analyze the proliferation of ventral meseneephalieprecursor cells(VMP). and celluar characteristics of the ventral meseneephalieprecursor from various gestational age mouse embryos are compared. Results:Neural stem cells of E11were cultured successfully in vitro with slower growth from1st to5th day, then geometric growth after that. They can divide, replicate andexpress nestin which is an embryo intermediate filament protein, considered as amarker of nerve stem cell. By the immunofluorescent staining not only ventralmeseneephalie precursor cells(VMP),but astrocytes and large amount of neurons canbe observed, compared with other gestational age embryonic cells,those age of11d-ays are better. Conclusion: Meseneephalie precursor cells of sprague dawley ratscan proliferate and differentiate in vitro, in which ventral meseneephalie precursorcells(VMP) of E11has the most capable of proliferation and differentiation. Thoughthis study more dopamine-producing neurons can be gained and then to be used celltransplantation therapy of the Neurodegenerative diseases, furthermore providing theoretic basis at cellular level for future research in vivo and clinical application andexperimental data.