The Anti-tumor Effect Of CP-31398in Androgen Independent Prostate Cancer Cells

Objective: To investigate the anti-tumor activity of CP-31398in androgenindependent prostate cancer in vitro and explore the possible mechanism.Methods: After DU-145cells were treated with1μg/mL,2μg/mL,4μg/mL,6μg/mL,8μg/mL and10μg/mL CP-31398, the cell activity was determined by MTT assay. Theexpression of p21were detected by RT-PCR on mRNA level, the expression of p53weredetected by Western-Blot on protein level. After CP-31398treatment, Flow cytometry(FCM) were performed to analysis the induction of apoptosis on tumor cells and the cellcycle of DU-145cells.Results: MTT assay results showed that the activity of cells decreased with higherconcentration of CP-31398. After the treatment of CP-31398， the percentage of apoptoticcells increased from (1.10persons0.53)%to (20.63±2.94)%(P<0.05); and thepercentage of G1/G0phase cells increased from (41.64±1.87)%to (54.72±1.45)%(p<0.05), while in S phase cells decreased from (44.95±2.23)%to (34.75±1.46)%(p<0.05), there was no significant changes in the G2/M phase cells.Conclusion: CP-31398could restore p53function in mutant p53-expressing cells,significantly inhibited the growth of androgen-independent prostate cancer cells in vitro,leading to cell cycle arrest and induction of apoptosis.