The Mechanism Of TMTP1-TAT-NBD’s Effects On Ovarian Cancer Cell Lines In Vitro

Objective: To investigate the mechanism of TMTP1-TAT-NBD’s effects on drug-resistantovarian cancer cell line C13K and its pairing-sensitive ovarian cancer cell line OV2008invitro, and to explore a new molecular targeted drugs for the suppression of ovarianmalignancy.Methods: After FITC-TMTP1effecting on ovarian cancer cell lines C13K and OV2008,use laser scanning confocal microscopy to observe the peptides affinity; after the effect ofTMTP1-TAT-NBD on ovarian cancer cell lines C13K and OV2008, the inhibition rate ofproliferation can be measured by MTT; the inhibition rate of colony can be measured bycolony formation; the rate of apoptosis was measured by flow cytometry. We carried out theWestern Blot to test the protein expression of NF-κB. NF-κB-activation and Nucleartranslocation assay can be taken to evaluate the effect of TMTP1-TAT-NBD on ovariancancer cell line C13K that inhibits the activation of NF-κB.Result: TMTP1can target the drug-resistant ovarian cancer cell line C13K, but it has poortarget to drug-sensitive ovarian cancer cell line OV2008. After TMTP1-TAT-NBD effectingon ovarian cancer cell lines C13K and OV2008, the proliferation of the cells mentionedabove was inhibited obviously (P<0.05); the colony formation of these cells was obviouslyinhibited (P<0.05); the apoptotic rate of the cell lines was raised (P<0.05). Meanwhile, therole of TMTP1-TAT-NBD has concentration-dependence, and the effects ofTMTP1-TAT-NBD on C13K were more obvious than that on OV2008. The proteinexpression level of NF-κB in cell line C13K which was showed by the Western Blot wasdown-regulated (P<0.05), however, the protein expression level of NF-κB in cell lineOV2008was not changed. NF-κB-activation and Nuclear translocation assay showedTMTP1-TAT-NBD could obviously inhibit the activation of NF-κB in ovarian cancer cellline C13K.Conclusion: TMTP1-TAT-NBD can be effective to ovarian cancer cell lines C13K and OV2008. The mechanism is it inhibit the activation of NF-κB. Therefore, it inhibits theovarian cancer cell lines’ proliferation and colony formation, and promotes apoptosis.