| Objective By observing the effect of Cynomorium Songaricum Polysaccharide (CSP) ongastric ulcer (GU),the pharmacological function of its anti-peptic ulcer(PU) is found. Throughthe usage of the modern technology and explanation of its mechanism in many ways, thestudy finds target and provides the experimental basis for clinical prevention PU and lays thefoundation for further research and development of the new drugs for anti-PU.Methods1.By using water-immersion stress, pyloric ligation, drug induced and acetic aciddamage four kinds of methods, to establish models of rat acute and chronic PU. Through thepreventive medicine and therapeutic medicine to stomach, to observe the effect of differentdosages of the CSP on index of GU of the rat.2.By observing the change of gastric juicequantity and the total acidity of rat of the type of pyloric ligation, and use the enzyme-linkedimmuno sorbent assay (ELISA) to detect Pepsin content in serum of the rat.3.By observingthe change of pathological morphology of the acetic acid damage type of rat and measured thethickness of the regeneration gastric mucous membrane and width of defect of mucousmuscularis of the rat, to detect superoxide dismutase (SOD) activity and malondialdehyde(MDA) content in serum of rat by the ultraviolet spectrophotometer.4.To testPlatelet-Activating Factor(PAF), Prostaglandin E2(PGE2) and Fpidermal Growth Factor(EGF)content in serum of the type of acetic acid damage of rat through the ELISA.Results1.Compared with the model group, there is significant difference, which the index ofstress type of rat in the high and moderate doses of CSP groups(P<0.01). There was noobvious difference between the high doses of CSP group and the Ranitidine group(P>0.05).2.The ulcer index of the pyloric ligation type of rat in the high and moderate doses of CSPgroups decline, which is significant difference to the model group(P<0.01). There was nodifference between the high doses of CSP group and the Ranitidine group(P>0.05), but thegastric juice quantity, the total acidity and the pepsin activity of the pyloric ligation type of ratin any doses of CSP groups are not obvious difference to the model group(P>0.05).3.Theulcer index of the indomethacin of rat in any doses of CSP groups are not obvious difference to the model group(P>0.05).4.Compared with the model group, the ulcer index of the aceticacid damage type in the high and moderate doses of CSP groups was significantlyreduced(P<0.01). There was no obvious difference between the high doses of CSP group andthe Ranitidine group(P>0.05).5.Observing the acetic acid damage type of rat by pathologicalhistology, the results show that the ulcer has healing of different degree in the high andmoderate doses of CSP groups. The area of ulcer is small, the depth is shallow and theadhesion is less, increasing the thickness of the regeneration mucous membrane, reduce thedefect width of the muscularis mucosa. It shows that the CSP can promote gastric epithelialtissue regeneration and improve scar tissue.6.The CSP can improve the activity of SOD inserum of the acetic acid damage type of the gastric ulcer rats and reduce the MDA content, toremove oxygen free radicals and to reduce the injury of the gastric mucosa.7.The CSP canimprove the EGF and PGE2content and reduce serum PAF content in serum and improve themucosa blood flow, strengthen the defensing and repairing function of the gastric mucosa, andpromote the ulcer healing.Conclusion The CSP has obvious protection and therapeutical effect on the GU rats of thestress type, pyloric ligation type and acetic acid damage type. Its mechanism may beconcerned with antioxidation ability, reducing the damage of the free radical, promotingendogenous the PGE2and EGF synthesis and release, inhibiting inflammatory mediators PAF,improving microcirculation of the gastric mucosa, and enhancing the defense and repairfunction of the gastric mucosa. However, the CSP has not the obvious function on the gastriculcer rats of indomethacin type. |
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