Regulation Of Multidrug Resistance And Invasive Growth By SATB1in Human Gastric Cancer Cells

Objective: Pecial AT-rich sequence binding protein1(SATB1) is anuclear factor that functions as the global chromatin organizer to regulategene expression. Recent evidence suggested the oncogenic role of SATB1in breast cancer. However, the role of SATB1in gastric cancer, especiallythe malignant phenotypes such as multi-drug resistance (MDR) andmetastasis remains poorly understood.Methods: In this study we used aggressive human gastric cancer cell lineSGC7901and its corresponding multi-drug resistant variantSGC7901/VCR cells as the model. RT-PCR and Western blot analysisshowed that SATB1was highly upregulated in SGC7901/VCR cells. Invitro drug sensitivity assay demonstrated a positive correlation betweenSATB1expression level and drug resistance.Results: Gain and loss of SATB1experiments further demonstrated thatSATB1contributes to MDR by inhibiting the accumulation of VCR ingastric cancer cells and protecting the cells from VCR induced apoptosis.In addition, we found that SATB1could promote the invasion ability ofgastric cancer cells.Conclusion: Our study provides novel insight into the oncogenic role ofSATB1in gastric cancer, suggesting that SATB1is a promising target forthe therapy of drug resistant and invasive gastric cancer.