Studies On The Protective Effect Of Extracellular Polysaccharide From Grifola Frondosa On Livre Injury

This paper focused research on exo-polysaccharide from Grifola frondosa (GFP), and three aspects were carried out. Firstly, the neutral sugar content was determined by phenol-sulfuric acid method, small molecule impurities were removed by ultrafiltration-percolation, three different molecular weight of GFP were obtained by ultrafiltration, which were GFP-1(MW10KD-30KD), GFP-2(MW30KD-50KD) and GFP-3(MW>5OKD). Secondly, This paper studied the protective effects in vitro of GFP on liver injury induced by CCL4. To determine which molecular weight range with a better protective effect on liver injury. The activities of alanine aminotransfe (ALT) and aspartate aminotransferase (AST), cell cycle, the expression detection of CYP2E1and TNF-a, fluorescence detection of ROS, and hematoxylin-eosin (H&E) straining were detected. The preliminary mechanism of liver injuried by CCL4were carried out. Thirdly, This paper studied the protective effect in vivo of GFP on liver injury induced by CCL4. After the establishment of the acute liver injury model of CCL4, The indexs of GFP effect on the liver injuried model induced by CCL4were detected, specific indicators were divided into two categories, the serum indicators was as the following:alanine aminotransfe, aspartate aminotransferase, Catalase, NO, Lipid-related indicators; another liver tissue indicators included:maleic dialdehyd, Hepatic glycogen, lactate dehydrogenase. Moreover, the histological changes of liver were observed, and liver fatty acid was carried out by Gas Chromatography.This paper studied the protective effect in vitro and in vivo of GFP on liver injury induced by CCL4. Experimental results showed that:GFP-2had much better protective effect of liver injury induced by CCL4, and GFP-2played important roles in preventing liver cells from being oxidized. GFP-2could reduce the activities of AST and ALT, thus releasing the cells to be arrested in S-phase, and decrease the expression of CYP2E1, TNF-a and the fluorescence of ROS. Besides, the addition of ROS inhibitors reduced the activities of AST and ALT. It could deeply explain that the protective effects of GFP-2was associated with the antioxidant effect. It is well known that arachidonic acid, EPA and other unsaturated fatty acids had played very important roles in the human body, so liver fatty acids was analized by Gas Chromatography in our study. Results showed that GFP could protected the unsaturated fatty acids in Liver cells not to be oxidized, which still was proved by the indicators determined in animal testing. Consequently, we clearly concluded that GFP possessed protective effect on acute hepatic injury induced by CCL4.